Li Mohan, Li Qilong, Dong Huaishuo, Zhao Shanshan, Ning Jianting, Bai Xue, Yue Xiqing, Xie Aijun
College of Food Science, Shenyang Agricultural University, Shenyang 110866, China.
College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
Int J Biol Macromol. 2022 Dec 1;222(Pt B):2628-2638. doi: 10.1016/j.ijbiomac.2022.10.045. Epub 2022 Oct 12.
Water-soluble polypeptides from pilose antler (PAWPs) are a traditional Chinese functional food and have been reported to inhibit triple-negative breast cancer (TNBC) in mice. Thus, in this study, we characterized PAWPs through peptidomics, and 405 total polypeptides were finally identified. Subsequently, our results indicate that PAWPs combined with neoadjuvant chemotherapy (NAC) result in a markedly lower spleen index compared with that in other groups. Next, 25 subpopulations of T cells were identified by multi-parametric flow cytometry in the lungs, spleen, and peripheral blood of different groups. These results indicated that PAWPs combined with NAC promote the proliferation of CD3 T cells in the spleen and significantly affect the fate of the T-cell subpopulation. Moreover, PAWPs combined with NAC increased the infiltration of CD4 interferon-γ T cells into tumor tissues. Our results showed that PAWPs have immunoregulatory functions and chemosensitizing effects, with good prospects for future clinical application.
鹿茸水溶性多肽（PAWPs）是一种传统的中国功能性食品，据报道可抑制小鼠三阴性乳腺癌（TNBC）。因此，在本研究中，我们通过肽组学对PAWPs进行了表征，最终鉴定出405种总多肽。随后，我们的结果表明，与其他组相比，PAWPs联合新辅助化疗（NAC）导致脾脏指数明显降低。接下来，通过多参数流式细胞术在不同组的肺、脾和外周血中鉴定出25个T细胞亚群。这些结果表明，PAWPs联合NAC促进脾脏中CD3 T细胞的增殖，并显著影响T细胞亚群的命运。此外，PAWPs联合NAC增加了CD4干扰素-γ T细胞向肿瘤组织的浸润。我们的结果表明，PAWPs具有免疫调节功能和化疗增敏作用，具有良好的未来临床应用前景。
