Department of Restorative Dentistry, Araraquara School of Dentistry, São Paulo State University -UNESP, Araraquara, SP, Brazil.
Department of Restorative Dentistry, Araraquara School of Dentistry, São Paulo State University -UNESP, Araraquara, SP, Brazil; Division of Endodontics, University of Minnesota, School of Dentistry, Minneapolis, MN, USA.
J Endod. 2022 Dec;48(12):1502-1510.e1. doi: 10.1016/j.joen.2022.09.010. Epub 2022 Oct 13.
The research for alternative irrigating solutions is ongoing, since no "ideal" solution has yet been found. Octenidine dihydrochloride (OCT) has been indicated as an endodontic irrigant because it has adequate antimicrobial and biological properties. The present study aimed to assess the effects of OCT on proliferation, migration, and induction of the osteogenic phenotype of stem cells from human dental pulp and apical papilla.
Cells were collected from human third molars and exposed to different doses of OCT, chlorhexidine (CHX), sodium hypochlorite (NaOCl), and ethylenediaminetetraacetic acid (EDTA) to determine cell viability by alamarBlue assay; proliferation by bromodeoxyuridine incorporation; migration by the Transwell assay; alkaline phosphatase activity by thymolphthalein release; and production of mineralized nodules by alizarin red staining. The results were analyzed by 1- or 2-way analysis of variance and Tukey (α = .05).
CHX promoted lower cell viability, followed by OCT, NaOCl, and EDTA, especially at intermediate doses (P < .05). Cells exposed to CHX had less proliferation than the other groups (P < .05). The Transwell assay revealed no differences among OCT, EDTA, and culture medium (control group) (P > .05). OCT and EDTA induced greater migration than CHX and NaOCl (P < .05). OCT and EDTA induced higher alkaline phosphatase activity than NaOCl and CHX (P < .05). No difference was detected among the groups using alizarin red staining (P > .05).
OCT induced high migration, proliferation, and alkaline phosphatase activity of stem cells from human dental pulp and apical papilla, which could be advantageous for regenerative endodontic procedures.
目前仍在研究替代冲洗液，因为尚未找到“理想”的溶液。洗必泰（CHX）已被认为是一种根管冲洗剂，因为它具有足够的抗菌和生物学特性。本研究旨在评估奥替尼啶二盐酸盐（OCT）对人牙髓和根尖乳头干细胞增殖、迁移和诱导成骨表型的影响。
从人第三磨牙中收集细胞，并将其暴露于不同剂量的 OCT、洗必泰（CHX）、次氯酸钠（NaOCl）和乙二胺四乙酸（EDTA），通过 alamarBlue 测定法测定细胞活力；通过溴脱氧尿苷掺入法测定增殖；通过 Transwell 测定法测定迁移；通过色原法测定碱性磷酸酶活性；通过茜素红染色测定矿化结节的产生。采用单因素或双因素方差分析和 Tukey 检验（α=0.05）进行结果分析。
CHX 促进细胞活力下降，其次是 OCT、NaOCl 和 EDTA，尤其是在中等剂量时（P<0.05）。与其他组相比，暴露于 CHX 的细胞增殖较少（P<0.05）。Transwell 测定法显示 OCT、EDTA 和培养基（对照组）之间无差异（P>0.05）。OCT 和 EDTA 诱导的迁移大于 CHX 和 NaOCl（P<0.05）。OCT 和 EDTA 诱导的碱性磷酸酶活性高于 NaOCl 和 CHX（P<0.05）。各组间茜素红染色无差异（P>0.05）。
OCT 诱导人牙髓和根尖乳头干细胞具有高迁移、增殖和碱性磷酸酶活性，这可能有利于再生性牙髓治疗。
