Baty J D, Rawle N W
J Chromatogr. 1987 Jun 12;395:395-402. doi: 10.1016/s0021-9673(01)94126-3.
A method for the analysis of the triacylglycerols from animal and human adipose tissue has been developed by combining high-performance liquid chromatography and gas-liquid chromatography (HPLC) and (GLC). Following isolation by thin-layer chromatography, the triacylglycerols are chromatographed by HPLC on a 5-micron Spherisorb ODS column with a gradient of 45 to 60% 2-propanol in acetonitrile as the mobile phase. Fractions eluted from the column are collected at intervals ranging from 0.2 to 1.0 min, and the triacylglycerols present are then trans-esterified prior to analysis of the resulting fatty acid methyl esters by an automated packed column GLC system. Triacylglycerols eluted from the column may be identified by a combination of their elution volume in HPLC and their fatty acid content. From the GLC data, profiles can be constructed for the distribution of several fatty acids amongst the triacylglycerols present in the adipose tissue sample. The method can be used with solvents such as acetonitrile, 2-propanol and acetone in the mobile phase, and there is no baseline drift during gradient elution. The adipose tissue triacylglycerols of normal and streptozotocin induced diabetic rats have been compared. No differences in the distribution of the fatty acids in the two groups of rats were observed under the conditions of our study.
通过结合高效液相色谱法（HPLC）和气液色谱法（GLC），已开发出一种分析动物和人体脂肪组织中三酰甘油的方法。通过薄层色谱法分离后，三酰甘油在5微米的Spherisorb ODS柱上进行HPLC色谱分析，以45%至60%的异丙醇在乙腈中的梯度溶液作为流动相。从柱中洗脱的馏分每隔0.2至1.0分钟收集一次，然后将存在的三酰甘油进行酯交换，再通过自动填充柱GLC系统分析所得脂肪酸甲酯。从柱中洗脱的三酰甘油可通过其在HPLC中的洗脱体积及其脂肪酸含量的组合来鉴定。根据GLC数据，可以构建脂肪组织样品中存在的几种脂肪酸在三酰甘油之间的分布图谱。该方法可使用乙腈、异丙醇和丙酮等溶剂作为流动相，在梯度洗脱过程中无基线漂移。已比较了正常大鼠和链脲佐菌素诱导的糖尿病大鼠的脂肪组织三酰甘油。在我们的研究条件下，未观察到两组大鼠脂肪酸分布的差异。
