Rawle N W, Willis R G, Baty J D
Dalgety PLC, Group Research Laboratory, Cambridge, UK.
Analyst. 1990 May;115(5):521-3. doi: 10.1039/an9901500521.
Triacylglycerols from rat adipose tissue were chromatographed by high-performance liquid chromatography (HPLC), with a gradient of propan-2-ol in acetonitrile as the mobile phase. Fractions of the material eluting from the column were collected and analysed by automated gas - liquid chromatography of the fatty acid methyl esters obtained after transmethylation. Triacylglycerols were identified by using a combination of their fatty acid content and elution time from the HPLC column. Fractions corresponding to whole peaks or groups of peaks were also collected and re-chromatographed on a liquid chromatography - mass spectrometry system equipped with a belt interface. For most triacylglycerols, good agreement was obtained between the two methods, although mass spectrometric identification of the early eluting peaks was complicated by poor resolution of the triacylglycerols on the HPLC system.
大鼠脂肪组织中的三酰甘油通过高效液相色谱（HPLC）进行色谱分析，以乙腈中异丙醇的梯度作为流动相。收集从柱中洗脱的物质馏分，并通过对甲基化后得到的脂肪酸甲酯进行自动气液色谱分析。通过结合三酰甘油的脂肪酸含量和从HPLC柱上的洗脱时间来鉴定三酰甘油。对应于整个峰或峰组的馏分也被收集，并在配备带式接口的液相色谱 - 质谱系统上重新进行色谱分析。对于大多数三酰甘油，两种方法之间获得了良好的一致性，尽管HPLC系统上三酰甘油的分辨率较差，使得早期洗脱峰的质谱鉴定变得复杂。
