RGB 颜色提取在水质毒性检测中的应用研究。
Application study of RGB color extraction in water toxicity detection.
机构信息
School of Chemistry and Environmental Engineering, Changchun University of Science and Technology, Changchun, Jilin 130022, PR China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China; University of Science and Technology of China, Hefei, Anhui, 230026, PR China.
出版信息
Bioelectrochemistry. 2023 Feb;149:108270. doi: 10.1016/j.bioelechem.2022.108270. Epub 2022 Oct 1.
In this work, a colorimetric biosensor based on the rapid color change of Escherichia coli DH 5α (E. coli) and p-benzoquinone (BQ) was introduced to evaluate water toxicity. Here, dark brown quinhydroquinone (QHQ) was obtained from the reaction between E. coli and BQ. The inhibition ratios were calculated by RGB (red, green, blue) values of samples or oxidation current of hydroquinone (HQ). Here, RGB technology was used for biotoxicity assessment for the first time. The results suggested that the biosensor-based RGB has an obvious dose-dependent response. The IC values of Hg and Cu obtained based on RGB values were 1.0 and 1.5 mg L, respectively. This biosensor can be used to detect quickly toxicity of the Cu and Hg in water without complicated operation steps. Therefore, the biosensor has potential application value in the field of water toxicity detection.
在这项工作中，引入了一种基于大肠杆菌 DH5α（E. coli）和对苯醌（BQ）快速变色的比色生物传感器来评估水毒性。在这里，深棕色的对苯二酚（QHQ）是由大肠杆菌和 BQ 之间的反应产生的。通过样品的 RGB（红、绿、蓝）值或对苯二酚（HQ）的氧化电流计算抑制率。在这里，RGB 技术首次被用于生物毒性评估。结果表明，基于生物传感器的 RGB 具有明显的剂量依赖性响应。基于 RGB 值获得的 Hg 和 Cu 的 IC 值分别为 1.0 和 1.5 mg/L。该生物传感器可用于快速检测水中的 Cu 和 Hg 的毒性，无需复杂的操作步骤。因此，该生物传感器在水毒性检测领域具有潜在的应用价值。
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