Sindelo Azole, Nene Lindokuhle, Nyokong Tebello
Institute of Nanotechnology Innovation, Rhodes University, PO Box 94, Makhanda 6140, South Africa.
Institute of Nanotechnology Innovation, Rhodes University, PO Box 94, Makhanda 6140, South Africa.
Photodiagnosis Photodyn Ther. 2022 Dec;40:103160. doi: 10.1016/j.pdpdt.2022.103160. Epub 2022 Oct 14.
The synthesis and characterization of neutral zinc and indium substituted mercaptobenzothiazole substituted phthalocyanines (Pcs) and their respective cationic derivatives are presented. The phthalocyanines were further covalently linked to two differently shaped amino-functionalized ZnO nanoparticles (ZnONPs): namely nanospheres (NH-ZnONSp), and nanopyramids (NH-ZnONPy), to form corresponding nanoconjugates. The photophysicochemical properties of each nanocomposite were determined, and the Pc-ZnONPs produced high singlet oxygen quantum yields. The photodynamic antimicrobial chemotherapy activity was determined using planktonic and biofilm cells of Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and Candida albicans (C. albicans). The conjugates of the cationic Pc derivatives with ZnONPy produced the highest log reduction values (∼ 8 and above) with the complete elimination of all planktonic cells at 0.45 kJ/cm for S. aureus and at 0.9 kJ/cm for E. coli, and C. albicans. For biofilms log reduction values >3 for both S. aureus and E. coli were obtained. The conjugates of the cationic Pc derivatives with NH-ZnONPy showed great potential in eradicating mixed microbial biofilms.
本文介绍了中性锌和铟取代的巯基苯并噻唑取代酞菁（Pcs）及其相应阳离子衍生物的合成与表征。这些酞菁进一步与两种不同形状的氨基官能化氧化锌纳米颗粒（ZnONPs）共价连接：即纳米球（NH-ZnONSp）和纳米金字塔（NH-ZnONPy），以形成相应的纳米共轭物。测定了每种纳米复合材料的光物理化学性质，并且酞菁-氧化锌纳米颗粒产生了较高的单线态氧量子产率。使用金黄色葡萄球菌（S. aureus）、大肠杆菌（E. coli）和白色念珠菌（C. albicans）的浮游细胞和生物膜细胞测定了光动力抗菌化疗活性。阳离子酞菁衍生物与ZnONPy的共轭物产生了最高的对数减少值（约8及以上），在0.45 kJ/cm²时金黄色葡萄球菌的所有浮游细胞以及在0.9 kJ/cm²时大肠杆菌和白色念珠菌的所有浮游细胞被完全消除。对于生物膜，金黄色葡萄球菌和大肠杆菌的对数减少值均>3。阳离子酞菁衍生物与NH-ZnONPy的共轭物在根除混合微生物生物膜方面显示出巨大潜力。
