Enan Gamal, Abdel-Shafi Seham, El-Nemr Mona, Shehab Wesam, Osman Ali, Sitohy Mahmoud, Sitohy Basel
Department of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig, Egypt.
Department of Chemistry, Faculty of Science, Zagazig University, Zagazig, Egypt.
Front Microbiol. 2023 Sep 26;14:1259334. doi: 10.3389/fmicb.2023.1259334. eCollection 2023.
The antibacterial and anti-biofilm activities of the 11S globulins isolated from lupin seeds (), and its methylated derivative (M11S), were investigated against seven pathogenic gram-positive and gram-negative bacteria. The MIC of 11S ranged from 0.1 to 4.0 μg/ml against 0.025 to 0.50 μg/ml for M11S, excelling some specific antibiotics. The MICs of M11S were 40-80 times lower than some specific antibiotics against gram-positive bacteria and 2-60 times lower than some specific antibiotics against gram-negative bacteria. One MIC of 11S and M11S highly reduced the liquid growth of all tested bacteria during 24 h at 37°C. They also inhibited biofilm formation by 80%-86% and 85%-94%, respectively (gram-positive), and 29%-44% and 43%-50%, respectively (gram-negative). M11S prevented biofilm formation by gram-positive bacteria at minimum biofilm inhibitory concentration (MBIC), 0.025-0.1 μg/ml against 0.1-0.5 μg/ml for gram-negative bacteria, i.e., 4-20 times and 4-7 times anti-biofilm inhibitory action compared with 11S, respectively. Biofilm formation of two bacteria revealed no adhered cells on glass slides for 24 h at 37°C, i.e., was entirely prevented by one MBIC of 11S and M11S. Scanning electron microscopy indicated microbial biofilm deformation under the action of 11S and M11S, indicating their broad specificity and cell membrane-targeted action.
对从羽扇豆种子中分离出的11S球蛋白（ ）及其甲基化衍生物（M11S）针对7种致病性革兰氏阳性和革兰氏阴性细菌的抗菌和抗生物膜活性进行了研究。11S的最低抑菌浓度（MIC）范围为0.1至4.0μg/ml，而M11S为0.025至0.50μg/ml，优于某些特定抗生素。M11S对革兰氏阳性细菌的MIC比某些特定抗生素低40 - 80倍，对革兰氏阴性细菌的MIC比某些特定抗生素低2 - 60倍。11S和M11S的一个MIC在37°C下24小时内显著降低了所有测试细菌的液体生长。它们还分别抑制了生物膜形成的80% - 86%和85% - 94%（革兰氏阳性），以及29% - 44%和43% - 50%（革兰氏阴性）。M11S在最低生物膜抑制浓度（MBIC）下阻止革兰氏阳性细菌形成生物膜，对革兰氏阴性细菌为0.025 - 0.1μg/ml，而11S为0.1 - 0.5μg/ml，即与11S相比，抗生物膜抑制作用分别高4 - 20倍和4 - 7倍。两种细菌的生物膜形成显示在37°C下24小时内载玻片上无粘附细胞，即完全被11S和M11S的一个MBIC阻止。扫描电子显微镜表明在11S和M11S作用下微生物生物膜变形，表明它们具有广泛的特异性和针对细胞膜的作用。
