基于组织微阵列荧光杂交技术分析结直肠癌患者 LINC01314 和 miR-96 的表达
Analysis of LINC01314 and miR-96 Expression in Colorectal Cancer Patients via Tissue Microarray-Based Fluorescence Hybridization.
机构信息
Department of Clinical Laboratory, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, China.
Department of Pathology, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, China.
出版信息
Dis Markers. 2022 Oct 7;2022:5378963. doi: 10.1155/2022/5378963. eCollection 2022.
METHODS
A tissue microarray (TMA) containing 76 individual colorectal tumor samples and 28 adjacent normal samples was constructed, and the expression levels of LINC01314 and miR-96 were detected by fluorescence in situ hybridization.
RESULTS
The expression levels of both LINC01314 and miR-96 were upregulated in CRC tissues and were associated with vascular metastasis ( < 0.05). A significantly positive correlation was observed between LINC01314 and miR-96 expression in tumor tissues ( < 0.001, = 0.870). Dominant expression of LINC01314 was a risk factor for both blood vessel invasion ( < 0.05) and poor 5-year survival ( = 0.001, hazard ratio = 4.144). The Kaplan-Meier analysis indicated that patients with LINC01314-dominant expression exhibited worse 5-year survival rates than those with miR-96-dominant expression ( < 0.05).
CONCLUSION
The expression patterns of both LINC01314 and miR-96 may be diagnostic of, and prognostic for, CRC. These findings will facilitate further exploration of the molecular mechanism of lncRNAs in CRC.
方法
构建了一个包含 76 个个体结直肠肿瘤样本和 28 个相邻正常样本的组织微阵列（TMA），并通过荧光原位杂交检测 LINC01314 和 miR-96 的表达水平。
结果
CRC 组织中 LINC01314 和 miR-96 的表达水平均上调，并与血管转移相关（<0.05）。肿瘤组织中 LINC01314 和 miR-96 的表达呈显著正相关（<0.001，=0.870）。LINC01314 的显性表达是血管侵犯的危险因素（<0.05）和 5 年生存率差的危险因素（=0.001，风险比=4.144）。Kaplan-Meier 分析表明，LINC01314 显性表达的患者 5 年生存率较 miR-96 显性表达的患者差（<0.05）。
结论
LINC01314 和 miR-96 的表达模式可能是 CRC 的诊断和预后标志物。这些发现将有助于进一步探索 lncRNA 在 CRC 中的分子机制。
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