Chen Xiaoming, Wang Kuan, Li Dazhuang, Zhao Mingyue, Huang Biao, Su Wenxing, Yu Daojiang
Department of Plastic and burns Surgery, The Second Affiliated Hospital of Chengdu Medical College (China National Nuclear Corporation 416 Hospital), Chengdu, China.
Department of Cosmetic Plastic and burns Surgery, The First Affiliated Hospital of Chengdu Medical College, Chengdu, China.
Front Genet. 2022 Sep 30;13:1038222. doi: 10.3389/fgene.2022.1038222. eCollection 2022.
Severe burns and blunt trauma can lead to multiple organ dysfunction syndrome, the leading cause of death in intensive care units. In addition to infection, the degree of immune inflammatory response also affects prognosis. However, the characteristics and clinical relevance of the common mechanisms of these major diseases are still underexplored. In the present study, we performed microarray data analysis to identify immune-related differentially expressed genes (DEGs) involved in both disease progression in burns and blunt trauma. Six analyses were subsequently performed, including gene enrichment analysis, protein-protein interaction (PPI) network construction, immune cell infiltration analysis, core gene identification, co-expression network analysis, and clinical correlation analysis. A total of 117 common immune-related DEGs was selected for subsequent analyses. Functional analysis emphasizes the important role of Th17 cell differentiation, Th1 and Th2 cell differentiation, Cytokine-cytokine receptor interaction and T cell receptor signaling pathway in these two diseases. Finally, eight core DEGs were identified using cytoHubba, including CD8A, IL10, CCL5, CD28, LCK, CCL4, IL2RB, and STAT1. The correlation analysis showed that the identified core DEGs were more or less significantly associated with simultaneous dysregulation of immune cells in blunt trauma and sepsis patients. Of these, the downregulation of CD8A and CD28 had a worse prognosis. Our analysis lays the groundwork for future studies to elucidate molecular mechanisms shared in burns and blunt trauma. The functional roles of identified core immune-related DEGs and dysregulated immune cell subsets warrant further in-depth study.
严重烧伤和钝性创伤可导致多器官功能障碍综合征,这是重症监护病房死亡的主要原因。除感染外,免疫炎症反应的程度也影响预后。然而,这些主要疾病共同机制的特征和临床相关性仍未得到充分探索。在本研究中,我们进行了微阵列数据分析,以鉴定参与烧伤和钝性创伤疾病进展的免疫相关差异表达基因(DEG)。随后进行了六项分析,包括基因富集分析、蛋白质-蛋白质相互作用(PPI)网络构建、免疫细胞浸润分析、核心基因鉴定、共表达网络分析和临床相关性分析。总共选择了117个常见的免疫相关DEG进行后续分析。功能分析强调了Th17细胞分化、Th1和Th2细胞分化、细胞因子-细胞因子受体相互作用以及T细胞受体信号通路在这两种疾病中的重要作用。最后,使用cytoHubba鉴定了八个核心DEG,包括CD8A、IL10、CCL5、CD28、LCK、CCL4、IL2RB和STAT1。相关性分析表明,鉴定出的核心DEG或多或少与钝性创伤和脓毒症患者免疫细胞的同时失调显著相关。其中,CD8A和CD28的下调预后较差。我们的分析为未来阐明烧伤和钝性创伤共同分子机制的研究奠定了基础。已鉴定的核心免疫相关DEG和失调的免疫细胞亚群的功能作用值得进一步深入研究。