Obidah Abert Habila, Umaru Aduwamai Hauwa, Shehu Adamu Saminu
Department of Biochemistry, Modibbo Adama University Yola, PMB 2076, Yola, Adamawa, Nigeria.
School of Science, Adamawa State College of Education Hong, Hong, Nigeria.
Biochem Res Int. 2022 Oct 7;2022:9311227. doi: 10.1155/2022/9311227. eCollection 2022.
The effect of iron oxide nanoparticles (FeONPs) synthesized using leaf extract on Triton X-100-induced atherosclerosis in white Wistar rats was determined. FeONPs were characterized to determine their size, structure, composition, and shape. antioxidant activity of FeONPs against 2, 2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) was determined. Atherosclerosis was induced by intraperitoneal administration of 5% Triton X-100 (100 mg/kg body weight) for 14 days. Group 1 received standard rat chow and water. Group 2 received 100 mg/kg body weight of Triton X-100 and a standard diet. Group 3 received 100 mg/kg body weight of Triton X-100 followed by 20 mg/kg body weight of atorvastatin for 21 days. Groups 4, 5, and 6 received 100 mg/kg body weight Triton X-100 was followed by variable concentrations of 100, 300, and 500 g/kg body weight FeONPs, respectively, for 21 days. Blood samples were analyzed for lipid, liver, antioxidant, and cardiovascular markers. Histopathology of the heart was also examined. Characterization revealed the amorphous nature, functional groups, and clustered topography of FeONPs. An upregulated antioxidant activity of FeONPs was observed in a dose-dependent manner. Administration of Triton X-100 showed elevated levels of lipid biomarkers except for high-density lipoprotein (HDL), which decreased in group 2 in comparison to group 1. Liver, antioxidant, and cardiovascular biomarkers all significantly increased. The structural alteration was observed in the heart tissue following histopathology examination. Administration of FeONPs significantly decreased all biomarkers and increased the level of HDL. Also, tissue architecture was restored. Our findings demonstrated that FeONPs were effective in ameliorating Triton X-100-induced atherosclerosis in rats.
测定了用树叶提取物合成的氧化铁纳米颗粒（FeONPs）对白色Wistar大鼠中由曲拉通X-100诱导的动脉粥样硬化的影响。对FeONPs进行了表征以确定其尺寸、结构、组成和形状。测定了FeONPs对2,2-二苯基-1-苦基肼基水合物（DPPH）的抗氧化活性。通过腹腔注射5%曲拉通X-100（100毫克/千克体重）持续14天诱导动脉粥样硬化。第1组给予标准大鼠饲料和水。第2组给予100毫克/千克体重的曲拉通X-100和标准饮食。第3组给予100毫克/千克体重的曲拉通X-100，随后给予20毫克/千克体重的阿托伐他汀，持续21天。第4、5和6组分别给予100毫克/千克体重的曲拉通X-100，随后分别给予100、300和500微克/千克体重的不同浓度FeONPs，持续21天。对血液样本进行脂质、肝脏、抗氧化和心血管标志物分析。还检查了心脏的组织病理学。表征揭示了FeONPs的无定形性质、官能团和聚集形貌。观察到FeONPs的抗氧化活性以剂量依赖性方式上调。给予曲拉通X-100后，除高密度脂蛋白（HDL）外，脂质生物标志物水平升高，与第1组相比，第2组的HDL降低。肝脏、抗氧化和心血管生物标志物均显著增加。组织病理学检查后在心脏组织中观察到结构改变。给予FeONPs后所有生物标志物均显著降低，HDL水平升高。此外，组织结构得以恢复。我们的研究结果表明，FeONPs在改善大鼠中由曲拉通X-100诱导的动脉粥样硬化方面是有效的。
