Department of Bioinformatics, Faculty of Technology, Marwadi University, Rajkot, Gujarat, India.
Department of Life Sciences, Biotechnology Division, School of Science, GSFC University, Vadodara-391750, Gujarat, India.
Iran Biomed J. 2024 Mar 1;28(2&3):71-81. doi: 10.61186/ibj.4061.
Bioreductive processes are quite potent, effective and affordable for the synthesis of green nanoparticles (NPs), as compared to the physical and chemical methods. The present study aimed to evaluate the bactericidal, antioxidative and anticancer activity of turmeric rhizome-iron oxide nanoparticles (FeONPs) derived from the turmeric rhizome (Curcuma amada) using ferric chloride as a precursor.
With focusing on the manufacture of FeONPs via green approach, we characterized the NPs using FTIR, FT-Vis, DLS, and UV-Vis spectroscopy. The produced particles were tested for antibacterial, antioxidant, and anticancer properties. The synthesized NPs were also examined using the MDA-MB-231 human epithelial breast cancer cell line and NCI-60 cancer cell lines.
The antioxidant activity of TR-FeONPs was concentration-dependent. The scavenging activity of TR-FeONPs was 76.09% at a concentration of 140 µg/ml. Using different concentrations of TR-FeONPs in the MTT assay against the MDA-MB-231 cell line indicated a reduction of less than 50% in cell viability at 125 µg/ml. Moreover, TR-FeONPs exhibited an effective bactericidal property. The gTR-FeONPs synthesized bioreductively were found to be effective in renal cancer, UO-31 cell line, with GI50 value of 66.64%.
Our study showcases a sustainable method based on green chemistry principles to produce FeONPs utilizing turmeric rhizome. We anticipate that the FeONPs produced through this biosynthesis process could serve as a promising drug delivery system in cancer treatment and as an effective antimicrobial agent against various diseases.
与物理和化学方法相比，生物还原过程对于绿色纳米粒子（NPs）的合成非常有效且具有成本效益。本研究旨在评估姜黄根茎-氧化铁纳米粒子（FeONPs）的杀菌、抗氧化和抗癌活性，该粒子由姜黄根茎（Curcuma amada）用氯化铁作为前体制备。
我们专注于通过绿色方法制造 FeONPs，使用 FTIR、FT-Vis、DLS 和 UV-Vis 光谱对 NPs 进行了表征。测试了所制备的粒子的抗菌、抗氧化和抗癌特性。还使用 MDA-MB-231 人上皮乳腺癌细胞系和 NCI-60 癌细胞系检查了合成的 NPs。
TR-FeONPs 的抗氧化活性呈浓度依赖性。TR-FeONPs 的清除活性在浓度为 140μg/ml 时为 76.09%。在 MTT 测定中，使用不同浓度的 TR-FeONPs 对 MDA-MB-231 细胞系进行测试，结果表明在 125μg/ml 时细胞活力降低不到 50%。此外，TR-FeONPs 表现出有效的杀菌特性。用生物还原法合成的 gTR-FeONPs 对肾癌细胞 UO-31 有效，GI50 值为 66.64%。
我们的研究展示了一种基于绿色化学原理的可持续方法，利用姜黄根茎生产 FeONPs。我们预计，通过这种生物合成过程生产的 FeONPs 可能成为癌症治疗中一种有前途的药物输送系统，并作为一种有效的抗菌剂对抗各种疾病。
