Clinical Research Center, Qingdao Municipal Hospital, Qingdao, China.
Department of Gastroenterology, Heilongjiang Provincial Hospital, Harbin, China.
Biomed Res Int. 2022 Oct 6;2022:8610467. doi: 10.1155/2022/8610467. eCollection 2022.
Coxsackievirus B (CVB) 3C protease (3C) plays a specific cleavage role on AU-rich binding factor (AUF1, also called hnRNP D), which consequently disputes the regulation of AUF1 on downstream molecules. In our study, the iTRAQ approach was first used to quantify the differentially expressed cellular proteins in AUF1-overexpressing HeLa cells, which provides straightforward insight into the role of AUF1 during viral infection. A total of 1,290 differentially expressed proteins (DEPs), including 882 upregulated and 408 downregulated proteins, were identified. The DEPs are involved in a variety of cellular processes via GO terms, protein-protein interactions, and a series of further bioinformatics analyses. Among the DEPs, some demonstrated important roles in cellular metabolism. In particular, DDX5 was further verified to be negatively regulated by AUF1 and increased in CVB-infected cells, which in turn promoted CVB replication. These findings provide potential novel ideas for exploring new antiviral therapy targets.
柯萨奇病毒 B3C 蛋白酶（3C）在富含 AU 的结合因子（AUF1，也称为 hnRNP D）上发挥特定的切割作用，从而影响 AUF1 对下游分子的调控。在我们的研究中，首先使用 iTRAQ 方法来定量分析 AUF1 过表达的 HeLa 细胞中的差异表达细胞蛋白，这为病毒感染期间 AUF1 的作用提供了直接的见解。总共鉴定到 1290 个差异表达蛋白（DEPs），包括 882 个上调和 408 个下调蛋白。通过 GO 术语、蛋白质-蛋白质相互作用和一系列进一步的生物信息学分析，这些 DEPs 参与了多种细胞过程。在 DEPs 中，一些在细胞代谢中显示出重要作用。特别是，DDX5 被进一步证实受到 AUF1 的负调控，并在 CVB 感染的细胞中增加，从而促进了 CVB 的复制。这些发现为探索新的抗病毒治疗靶点提供了潜在的新想法。
