Department of Basic Medicine, Changsha Health Vocational College, Changsha, 410600 Hunan, China.
Hunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine, Hunan University of Medicine, Huaihua, 418000 Hunan, China.
Biomed Res Int. 2022 Oct 7;2022:8453011. doi: 10.1155/2022/8453011. eCollection 2022.
To investigate the effect and mechanism of resveratrol on the biological behavior of cervical cancer cells (HeLa cells), the apoptosis, migration, and invasion of HeLa cells were detected by flow cytometry, wound healing, and transwell assays. The expression levels of Hedgehog signal pathway proteins (smoothened (SMO), zinc finger transcription factors (Gli1), and sonic hedgehog homolog (Shh)) were detected by quantitative real-time PCR (qPCR) and western blotting. Compared with that control group, resveratrol (RES) significantly induced apoptosis, inhibited the migration and invasion of the HeLa cells. The expression of SMO, Gli1, and Shh were downregulated in the HeLa cells treated with RES. The Hedgehog agonist purmorphamine (PUR) reversed the RES-induced increase of apoptosis and reduction of migration and invasion in the HeLa cells. In conclusion, RES induced the apoptosis and suppressed the migration and invasion of HeLa cells by inhibiting Hedgehog signal pathway.
为了研究白藜芦醇对宫颈癌（HeLa 细胞）生物学行为的影响及作用机制，通过流式细胞术、划痕愈合实验和 Transwell 实验检测 HeLa 细胞的凋亡、迁移和侵袭，通过实时定量 PCR（qPCR）和蛋白质印迹法检测 Hedgehog 信号通路蛋白（ smoothened（SMO）、锌指转录因子（Gli1）和 sonic hedgehog 同源物（Shh））的表达水平。与对照组相比，白藜芦醇（RES）显著诱导细胞凋亡，抑制 HeLa 细胞的迁移和侵袭。RES 处理后的 HeLa 细胞中 SMO、Gli1 和 Shh 的表达下调。 Hedgehog 激动剂 purmorphamine（PUR）逆转了 RES 诱导的细胞凋亡增加和迁移及侵袭减少。结论：RES 通过抑制 Hedgehog 信号通路诱导 HeLa 细胞凋亡，抑制其迁移和侵袭。
