耐缺氧胰岛素生成细胞在改善糖尿病方面的潜在作用。
The promising role of hypoxia-resistant insulin-producing cells in ameliorating diabetes mellitus .
作者信息
Ahmed Hanaa H, Aglan Hadeer A, Beherei Hanan H, Mabrouk Mostafa, Mahmoud Nadia S
机构信息
Hormones Department, Medical Research & Clinical Studies Institute, National Research Centre, Giza, 12622, Egypt.
Stem Cells Lab, Center of Excellence for Advanced Sciences, National Research Centre, Giza, 12622, Egypt.
出版信息
Future Sci OA. 2022 Sep 14;8(7):FSO811. doi: 10.2144/fsoa-2022-0005. eCollection 2022 Aug.
AIM
This study aimed to evaluate the efficacy of hypoxia-persistent insulin-producing cells (IPCs) against diabetes .
MATERIALS & METHODS: Mesenchymal stem cells (MSCs) differentiation into IPCs in the presence of Se/Ti (III) or CeO nanomaterials. IPCs were subjected to hypoxia and hypoxia genes were analyzed. PKH-26-labeled IPCs were infused in diabetic rats to evaluate their anti-diabetic potential.
RESULTS
MSCs were differentiated into functional IPCs. IPCs exhibited overexpression of anti-apoptotic genes and down-expression of hypoxia and apoptotic genes. IPCs implantation elicited glucose depletion and elevated insulin, HK and G6PD levels. They provoked and upregulation and and down-regulation. IPCs transplantation ameliorated the destabilization of pancreatic tissue architecture.
CONCLUSION
The chosen nanomaterials were impressive in generating hypoxia-resistant IPCs that could be an inspirational strategy for curing diabetes.
目的
本研究旨在评估缺氧持续存在的胰岛素产生细胞（IPCs）对糖尿病的疗效。
材料与方法
间充质干细胞（MSCs）在硒/三价钛（Se/Ti(III)）或二氧化铈（CeO）纳米材料存在的情况下分化为IPCs。对IPCs进行缺氧处理并分析缺氧基因。将PKH-26标记的IPCs注入糖尿病大鼠体内以评估其抗糖尿病潜力。
结果
MSCs分化为功能性IPCs。IPCs表现出抗凋亡基因的过表达以及缺氧和凋亡基因的下调。IPCs植入引起血糖消耗以及胰岛素、己糖激酶（HK）和葡萄糖-6-磷酸脱氢酶（G6PD）水平升高。它们引发了……的上调和……的下调。IPCs移植改善了胰腺组织结构的不稳定。
结论
所选用的纳米材料在生成抗缺氧的IPCs方面效果显著，这可能是一种治疗糖尿病的启发式策略。
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