Tavakolpournegari Alireza, Hashemi Mehrdad, Zare Karizi Shohreh, Matin Ahmadi Arash, Bidooki Seyed Hesamoddin, Banaei Gooya
Group of Mutagenesis, Department of Genetics and Microbiology, Faculty of Science and Bioscience, Autonomous University of Barcelona, Bellaterra, Spain.
Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Iran J Public Health. 2022 Jul;51(7):1594-1601. doi: 10.18502/ijph.v51i7.10093.
One of the important molecular pathways in breast cancer is the PTEN-PI3K-AKT pathway. Any change in the activity of the PTEN gene can alter the PI3K-AKT pathway. Moreover, there are subsets of genes and pathways their expression changes by post-transcriptional regulations. For instance, gene regulation alters by non-coding RNAs such as micro-RNAs as post-transcriptional regulators that prevent the expression of the target transcript. Therefore, it is essential to assess the related alterations in micro-RNA expression patterns to find out the possible causes of conversions in related transcripts and pathways such as the PTENPI3K-AKT pathway in breast cancer.
To determine the expression level of miR-181a and miR-30d in 30 breast tumor samples and 30 adjacent normal samples, the RNA extraction, and cDNA synthesis was performed by RiboEx (GeneAll, Korea). Finally, the Real-Time PCR method was used for quantitative analysis of the expression levels of these miRNAs. all the experimental part of the project in done at Islamic Azad University in 2017.
After analyzing comparisons in the expression level of miR-181a and miR-30d in tumor and normal tissues, there was a significant increase in the expression level of miR-181a in tumor samples compared with normal samples. Moreover, the expression level of miR-30d in tumor samples reported a significant decrease in comparison with normal samples (<0.05).
Upregulation of miR-181a may affect the transcription of the PTEN gene resulting in the cell progress to cancer. The Downregulation of miR-30d may also lead to cancer cell growth, due to a reduction in the affecting on the CREB gene transcript.
乳腺癌中重要的分子途径之一是PTEN-PI3K-AKT途径。PTEN基因活性的任何变化都可改变PI3K-AKT途径。此外，存在一些基因和途径的子集，其表达通过转录后调控发生变化。例如，基因调控可由非编码RNA（如微小RNA）作为转录后调节因子来改变，这些因子可阻止靶转录本的表达。因此，评估微小RNA表达模式的相关改变对于找出相关转录本和途径（如乳腺癌中的PTEN-PI3K-AKT途径）转化的可能原因至关重要。
为了确定30例乳腺肿瘤样本和30例相邻正常样本中miR-181a和miR-30d的表达水平，使用RiboEx（韩国GeneAll公司）进行RNA提取和cDNA合成。最后，采用实时PCR方法对这些miRNA的表达水平进行定量分析。该项目的所有实验部分于2017年在伊斯兰阿扎德大学完成。
在分析肿瘤组织和正常组织中miR-181a和miR-30d的表达水平比较后发现，与正常样本相比，肿瘤样本中miR-181a的表达水平显著升高。此外，与正常样本相比，肿瘤样本中miR-30d的表达水平显著降低（<0.05）。
miR-181a的上调可能影响PTEN基因的转录，导致细胞发展为癌症。miR-30d的下调也可能由于对CREB基因转录本的影响减少而导致癌细胞生长。
