电子显微镜放射自显影中的分辨率V：45钙（和33磷）。
Resolution in EM autoradiography V: 45Ca (and 33P).
作者信息
Salpeter M M, Nelson R, Harris R, Salpeter E E
出版信息
J Histochem Cytochem. 1987 Oct;35(10):1047-51. doi: 10.1177/35.10.3624849.
PMID:3624849
Abstract
We devised a specimen to test EM autoradiographic resolution for calcium-45 (and phosphorous-33). A dry emulsion film was used for soluble compounds. We obtained a resolution (HD) value of 2300 +/- 500 A. This value is no larger than that previously obtained for Carbon -14, whose emission is about 1.6-fold lower. This result is as expected on theoretical grounds.
摘要
我们设计了一种标本，用于测试钙-45（和磷-33）的电子显微镜放射自显影分辨率。对于可溶性化合物，使用了干乳胶膜。我们获得的分辨率（HD）值为2300±500埃。该值不大于先前获得的碳-14的分辨率值，碳-14的发射强度约低1.6倍。从理论角度来看，这个结果是预期的。
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