Hamburger A W, Lurie K, Condon M E
Int J Cell Cloning. 1987 Jul;5(4):347-55. doi: 10.1002/stem.5530050409.
Human peripheral blood monocytes release a factor that enhances the clonal growth of human epithelial tumor cells in soft agar. The monocyte-derived growth factor was needed for both cellular proliferation and survival. Survival of SW-13 colony-forming cells decreased linearly in the absence of monocyte-conditioned media (MO-CM). Cells failed to respond to MO-CM after four days in culture. Although MO-CM enhanced growth when cells were plated at low density, growth was also enhanced when cell density was not a limiting factor. MO-CM increased DNA synthesis of SW-13 cells growing in monolayer culture as measured by tritiated-thymidine incorporation. These findings support evidence indicating inflammatory products may play a role in maintenance of the transformed phenotype.
人外周血单核细胞释放一种因子，该因子可增强人上皮肿瘤细胞在软琼脂中的克隆生长。单核细胞衍生的生长因子对于细胞增殖和存活均是必需的。在没有单核细胞条件培养基（MO-CM）的情况下，SW-13集落形成细胞的存活率呈线性下降。培养四天后，细胞对MO-CM不再有反应。尽管当细胞以低密度接种时MO-CM可促进生长，但当细胞密度不是限制因素时，生长也会增强。通过氚标记胸腺嘧啶核苷掺入法测定，MO-CM增加了单层培养的SW-13细胞的DNA合成。这些发现支持了炎症产物可能在维持转化表型中起作用的证据。
