Halper J, Moses H L
Cancer Res. 1983 May;43(5):1972-9.
SW-13 cells, derived from a human adenocarcinoma of the adrenal cortex, formed only a few small colonies when suspended in soft agar at low cell densities. The number and size of colonies increased dramatically following stimulation with serum-free medium conditioned by SW-13 cells, indicating the possibility of autostimulation in these malignant cells. Evidence is presented suggesting that SW-13 cells form progressively growing soft agar colonies upon stimulation by epithelial tissue-derived growth factor-like polypeptides. Both acid-ethanol extracts and conditioned media from three human carcinoma cell lines (A431, D562, and A549) caused similar increases in colony number and size of SW-13 cells. Extracts from 26 of 32 freshly excised human carcinomas and five freshly excised nonneoplastic human kidneys and one human lung stimulated soft agar growth of SW-13 cells as well. None of the nine extracts from nonepithelial human solid malignant tumors stimulated SW-13 cells. However, a benign nonepithelial tumor (uterine leiomyoma) caused a low level of soft agar growth of SW-13 cells. Cell extract from A204 human sarcoma cells and both conditioned medium and acid-ethanol cell extract from A375 human melanoma cells lacked SW-13 activity, whereas medium conditioned by A204 cells stimulated soft agar growth of SW-13 cells. Chemical and physical treatment data indicated that the epithelial tissue-derived growth factor-like substances are acid- and heat-stable polypeptides with disulfide bonds. The major peak of this activity had an apparent molecular weight of 20,000 to 22,000 and was clearly separable from transforming growth factors reported previously which stimulate colony formation by nontransformed mouse AKR-2B and rat NRK cells. The major peaks of SW-13, NRK, and AKR-2B activity could be separated by high-performance liquid chromatography. This SW-13 activity induced irreversible anchorage-independent growth of SW-13 cells and an increase in DNA synthesis as measured by [3H]thymidine incorporation.
SW - 13细胞源自人类肾上腺皮质腺癌，当以低密度悬浮于软琼脂中时，仅形成少数小菌落。用SW - 13细胞条件无血清培养基刺激后，菌落数量和大小显著增加，表明这些恶性细胞存在自刺激的可能性。有证据表明，SW - 13细胞在受到上皮组织衍生生长因子样多肽刺激后形成逐渐生长的软琼脂菌落。来自三种人类癌细胞系（A431、D562和A549）的酸乙醇提取物和条件培养基也使SW - 13细胞的菌落数量和大小出现类似增加。32例新鲜切除的人类癌组织中的26例以及5例新鲜切除的非肿瘤性人类肾脏和1例人类肺组织的提取物也刺激了SW - 13细胞在软琼脂中的生长。来自9例非上皮性人类实体恶性肿瘤的提取物均未刺激SW - 13细胞。然而，一种良性非上皮性肿瘤（子宫平滑肌瘤）使SW - 13细胞在软琼脂中的生长水平较低。A204人肉瘤细胞的细胞提取物以及A375人黑色素瘤细胞的条件培养基和酸乙醇细胞提取物均缺乏SW - 13活性，而A204细胞条件培养基刺激了SW - 13细胞在软琼脂中的生长。化学和物理处理数据表明，上皮组织衍生生长因子样物质是具有二硫键的酸稳定和热稳定多肽。该活性的主要峰的表观分子量为20,000至22,000，并且与先前报道的刺激未转化的小鼠AKR - 2B和大鼠NRK细胞形成菌落的转化生长因子明显可分离。SW - 13、NRK和AKR - 2B活性的主要峰可通过高效液相色谱法分离。这种SW - 13活性诱导SW - 13细胞不可逆的不依赖贴壁生长，并通过[³H]胸苷掺入法测量的DNA合成增加。
