印度中央邦丁多里区以及恰蒂斯加尔邦坎克尔和巴斯塔尔地区拟除虫菊酯抗性强度生物测定的首次报告。

First Report on Intensity Bioassays for Pyrethroid Resistance in . in District Dindori of Madhya Pradesh State and Districts Kanker and Bastar of Chhattisgarh State, India.

作者信息

Mishra Ashok K, Singh Mrigendra P, Das Aparup, Raghavendra Kamaraju

机构信息

ICMR-National Institute of Research in Tribal Health (NIRTH), NIRTH Campus, Medical, P. O Garha, Jabalpur, Madhya Pradesh, India.

ICMR-National Institute of Malaria Research, Sector 8, Dwarka, New Delhi, India.

出版信息

J Trop Med. 2022 Sep 20;2022:1595604. doi: 10.1155/2022/1595604. eCollection 2022.

DOI:10.1155/2022/1595604

PMID:36249735

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9553532/

Abstract

BACKGROUND

The major malaria vector in India is , and indoor residual spraying (IRS) and distribution of long-lasting insecticidal nets (LLINs) are the two main vector control measures in the national program. This species has shown resistance to dichlorodiphenyltrichloroethane (DDT), malathion, and synthetic pyrethroids (SP). The study was carried out in three districts, that is, Dindori of Madhya Pradesh and Kanker and Bastar from Chhattisgarh state to know the range of resistance phenotypes and to assess the strength of resistance in . .

METHODS

. collected from the field was tested to determine the susceptibility status to the discriminating concentration (DC) of different insecticides, that is, DDT 4.0%, malathion 5.0%, alphacypermethrin 0.05%, and deltamethrin 0.05% following the World Health Organization (WHO) procedures. Further, intensity bioassays of the resistant . to 1X discriminating concentration (DC) of alphacypermethrin and deltamethrin were conducted by exposing 5X and 10X concentrations of 1X DC. Results are interpreted as per the WHO criterion.

RESULTS

The overall result of susceptibility status in . in districts Dindori, Kanker, and Bastar revealed confirmed resistance to DDT, malathion, alphacypermethrin, and deltamethrin registering mortality of 15% (10-20%), 70% (65-75%), 78.6% (77-82.5%), and 87% (84.8-91.3%), respectively. Further, in district Dindori and Baster, the intensity bioassay test at 5X DC of alphacypermethrin and deltamethrin revealed 99% and 100%, respectively, while in district Kanker, the species registered moderate resistance with 92.5% and 95% mortality, respectively, in 5X DC of alphacypermethrin and deltamethrin. However, in 10X DC, the . was susceptible to both pyrethroids (100%) in district Kanker.

CONCLUSION

The results of the intensity bioassay tests with SP-resistant . suggested no change of insecticide is required in the ongoing intervention. However, regular monitoring of insecticide susceptibility and intensity bioassays in malaria vectors in view of continued use of these interventions may increase resistance and for implementing effective vector management strategies.

摘要

背景

印度主要的疟疾传播媒介是[媒介名称未给出]，室内滞留喷洒（IRS）和长效驱虫蚊帐（LLINs）的分发是国家项目中的两项主要病媒控制措施。该物种已对滴滴涕（DDT）、马拉硫磷和拟除虫菊酯（SP）表现出抗性。该研究在三个地区开展，即中央邦的丁多里以及恰蒂斯加尔邦的坎克尔和巴斯塔尔，以了解抗性表型范围并评估[媒介名称未给出]的抗性强度。

方法

按照世界卫生组织（WHO）的程序，对从野外采集的[媒介名称未给出]进行测试，以确定其对不同杀虫剂鉴别浓度（DC）的易感性状况，即4.0%的滴滴涕、5.0%的马拉硫磷、0.05%的高效氯氰菊酯和0.05%的溴氰菊酯。此外，通过暴露5倍和10倍浓度的1倍鉴别浓度（DC），对具有抗性的[媒介名称未给出]进行高效氯氰菊酯和溴氰菊酯1倍鉴别浓度（DC）的强度生物测定。结果根据WHO标准进行解读。

结果

丁多里、坎克尔和巴斯塔尔地区[媒介名称未给出]的易感性总体结果显示，对滴滴涕、马拉硫磷、高效氯氰菊酯和溴氰菊酯均存在确诊抗性，死亡率分别为15%（10 - 20%）、70%（65 - 75%）、78.6%（77 - 82.5%）和87%（84.8 - 91.3%）。此外，在丁多里和巴斯塔尔地区，高效氯氰菊酯和溴氰菊酯5倍鉴别浓度（DC）的强度生物测定试验分别显示死亡率为99%和100%，而在坎克尔地区，该物种在高效氯氰菊酯和溴氰菊酯5倍鉴别浓度（DC）下分别表现出中度抗性，死亡率为92.5%和95%。然而，在10倍鉴别浓度（DC）下，坎克尔地区的[媒介名称未给出]对两种拟除虫菊酯均敏感（100%）。