He Shuangjun, Yang Jiani, Fan Xinxin, Lu Dong, Zhang Shuang, Yan Lei
91625Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Argo-Waste in Cold Region, College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, PR China.
53045Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, China.
J Biomater Appl. 2023 Feb;37(7):1325-1338. doi: 10.1177/08853282221133647. Epub 2022 Oct 17.
Bacterial magnetosomes had been proved to have great application potential in medicine and biotechnology. The objective of the present study was to obtain high yield of magnetosomes from () BYM in an airlift bioreactor using response surface methodology (RSM). The magnetosomes from BYM were characterized using a transmission electron microscope and scanning electron microscopy. The maximum magnetosome yield of 0.4267 mg/L was achieved at ventilation capacity of 3.6 L/min and gluconic acid concentration of 10 mmol/L at 25C. The correlation coefficient (R) value of 0.8676 of the obtained model suggested a good correlation between the actual and predicted magnetosome yield. The confirmation experiment confirmed that the actual magnetosome yield of 0.391 mg/L obtained were in agreement with the predicted value of 0.398 mg/L. These results suggested that RSM can be employed to find out the optimum conditions for magnetosome formation in airlift bioreactor.
细菌磁小体已被证明在医学和生物技术领域具有巨大的应用潜力。本研究的目的是使用响应面法（RSM）在气升式生物反应器中从（）BYM获得高产率的磁小体。使用透射电子显微镜和扫描电子显微镜对来自BYM的磁小体进行表征。在25℃下，通气量为3.6 L/min、葡萄糖酸浓度为10 mmol/L时，磁小体的最大产量达到0.4267 mg/L。所获得模型的相关系数（R）值为0.8676，表明实际磁小体产量与预测产量之间具有良好的相关性。验证实验证实，获得的实际磁小体产量为0.391 mg/L，与预测值0.398 mg/L一致。这些结果表明，响应面法可用于找出气升式生物反应器中磁小体形成的最佳条件。
