de Carvalho Maryana Hermínio, de Araújo Hallysson Douglas Andrade, da Silva Renata Pereira, Dos Santos Correia Maria Tereza, de Freitas Katia Cristina Silva, de Souza Sandra Rodrigues, Barroso Coelho Luana Cassandra Breitenbach
Departamento de Bioquímica, Centro de Biociências, CB, Universidade Federal de Pernambuco, Avenida Prof. Moraes Rego, 1235, Cidade Universitária, 50670-420, Recife-PE, Brazil.
Departamento de Química, Universidade Federal Rural de Pernambuco, UFRPE, Rua Dom Manuel de Medeiros, S/N, Dois Irmãos, Recife-PE, 52171-900, Brazil.
Chem Biodivers. 2022 Dec;19(12):e202200515. doi: 10.1002/cbdv.202200515. Epub 2022 Nov 10.
Biosensors are small devices known for their selectivity, high specificity and sensitivity to the respective analyte, at low concentrations. We developed an electrochemical biosensor using the crystalline polymer MOF-[Cu (BTC) (H O) ] to characterize Cratylia mollis seed lectin (Cramoll) and its interaction with free carbohydrate (glucose) and carbohydrates on the surface of rabbit erythrocytes. The electrochemical potentials presented by the exponential curves that vary from 96 to 142 mV in relation to concentrations of 10 to 20 mM of glucose are decisive for the use of the system containing gold electrode/MOF/Cramoll for the characterization of biological models due to its high sensitivity. As well as the kinetic behavior presented in the cyclic voltammograms, with a cathodic current response of 0.000 3 A for a glucose concentration of 15 mM. These results were due to the high specificity of Cramoll under these conditions, promoting stability of surface charges at the Cramoll/electrode interface. This phenomenon facilitates the monitoring of the interaction with free glucose present in the electrolyte medium by potentiometric and amperometric methods and with carbohydrates present on the surface of rabbit erythrocytes through the potentiometric method. Through scanning electron microscopy (SEM) it was possible to observe Cramoll immobilized on the MOF surface, proving the specificity of the ligand (glucose-lectin) through the morphological lectin changes in this process. This electrochemical model, Cramoll/MOF biosensor, is effective for evaluating free lectin/carbohydrate or in the erythrocyte membrane.
生物传感器是一种小型设备，以其对低浓度下各自分析物的选择性、高特异性和敏感性而闻名。我们开发了一种使用结晶聚合物MOF-[Cu (BTC) (H₂O)₂]的电化学生物传感器，以表征毛蟹爪兰种子凝集素（Cramoll）及其与游离碳水化合物（葡萄糖）以及兔红细胞表面碳水化合物的相互作用。对于10至20 mM葡萄糖浓度，指数曲线呈现的电化学电位在96至142 mV之间变化，由于其高灵敏度，对于使用包含金电极/MOF/Cramoll的系统来表征生物模型具有决定性意义。以及循环伏安图中呈现的动力学行为，对于15 mM的葡萄糖浓度，阴极电流响应为0.000 3 A。这些结果归因于在这些条件下Cramoll的高特异性，促进了Cramoll/电极界面处表面电荷的稳定性。这种现象便于通过电位法和安培法监测与电解质介质中游离葡萄糖的相互作用，以及通过电位法监测与兔红细胞表面碳水化合物的相互作用。通过扫描电子显微镜（SEM）可以观察到Cramoll固定在MOF表面，通过该过程中凝集素形态的变化证明了配体（葡萄糖-凝集素）的特异性。这种电化学生物传感器模型，即Cramoll/MOF生物传感器，对于评估游离凝集素/碳水化合物或红细胞膜中的情况是有效的。
