Hainan Eye Hospital and Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Haikou, Hainan Province, China.
Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Medicine (Baltimore). 2022 Oct 14;101(41):e31048. doi: 10.1097/MD.0000000000031048.
To compare the concentrations of protein markers in aqueous humor (AH) of patients with primary open-angle glaucoma (POAG), chronic angle-closure glaucoma (CACG), acute primary angle closure (APAC), and cataract without glaucoma as the control group. AH samples were collected at the beginning of surgery from 82 eyes of 82 patients who were divided into POAG (n = 23), CACG (n = 21), APAC (n = 19), and cataract groups (n = 19). The expression levels of interferon-gamma (IFN-γ), interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-17A (IL-17A), lymphotoxin-alpha (LT-α), monocyte chemotactic protein-1 (MCP-1), matrix metalloproteinase-2 (MMP-2), brain derived neurotrophic factor (BDNF), basic fibroblast growth factor (bFGF), platelet-derived growth factor-AA (PDGF-AA), vascular endothelial growth factor (VEGF), tissue inhibitor of metalloproteinases-1 (TIMP-1), and tumor necrosis factor-alpha (TNF-α) in AH were detected using a microsphere-based immunoassay. The AH levels of TNF-α, MMP-2, MCP-1, IFN-γ, and TIMP-1 in the APAC and CACG groups were significantly higher than those in control eyes. Additionally, the AH levels of interleukin-6 (IL-6) and VEGF in the APAC group were significantly higher than those in the control group (CG). The interleukin-8 (IL-8) levels in patients with POAG were significantly higher than those in control eyes, whereas the LT-α levels were significantly lower than those in control eyes. IL-6 levels were significantly correlated with the coefficient of variation (CV), whereas IL-6 levels were significantly negatively correlated with the frequency of hexagonal cells (HEX) and corneal endothelial cell density (CD). The levels of TNF-α, MMP-2, MCP-1, IFN-γ, TIMP-1, IL-6, IL-8, VEGF, and LT-α were different among the three types of glaucoma. These different types of glaucoma may be caused by various pathogeneses, which opens avenues for further investigation into the pathogenesis of glaucoma and discoveries new targets and pathways for the treatment of glaucoma.
比较原发性开角型青光眼（POAG）、慢性闭角型青光眼（CACG）、急性原发性闭角型青光眼（APAC）和白内障无青光眼患者房水中蛋白标志物的浓度。从 82 名患者的 82 只眼中收集房水样本，这些患者分为 POAG（n = 23）、CACG（n = 21）、APAC（n = 19）和白内障组（n = 19）。采用微球免疫分析检测房水中干扰素-γ（IFN-γ）、白细胞介素-2（IL-2）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、白细胞介素-17A（IL-17A）、淋巴毒素-α（LT-α）、单核细胞趋化蛋白-1（MCP-1）、基质金属蛋白酶-2（MMP-2）、脑源性神经营养因子（BDNF）、碱性成纤维细胞生长因子（bFGF）、血小板衍生生长因子-AA（PDGF-AA）、血管内皮生长因子（VEGF）、金属蛋白酶组织抑制剂-1（TIMP-1）和肿瘤坏死因子-α（TNF-α）的表达水平。APAC 和 CACG 组房水中 TNF-α、MMP-2、MCP-1、IFN-γ和 TIMP-1 水平明显高于对照组。此外，APAC 组房水中白细胞介素-6（IL-6）和 VEGF 水平明显高于对照组。POAG 患者白细胞介素-8（IL-8）水平明显高于对照组，而 LT-α 水平明显低于对照组。IL-6 水平与变异系数（CV）显著相关，而 IL-6 水平与六方细胞（HEX）频率和角膜内皮细胞密度（CD）显著负相关。三种青光眼之间 TNF-α、MMP-2、MCP-1、IFN-γ、TIMP-1、IL-6、IL-8、VEGF 和 LT-α 水平不同。这些不同类型的青光眼可能由不同的发病机制引起，为进一步研究青光眼的发病机制和发现青光眼治疗的新靶点和途径开辟了道路。
