Peng Jun, Wang Shuhan, Liu Wenliang, Ke Haoqi, Gao Xiaofeng
Shenzhen Institute for Drug Control (Shenzhen Testing Center of Medical Devices), Shenzhen, 518057.
Zhongguo Yi Liao Qi Xie Za Zhi. 2022 Sep 30;46(5):560-564. doi: 10.3969/j.issn.1671-7104.2022.05.017.
To characterize the silver in chitosan antibacterial gel, and to establish a method for the determination of silver content in samples.
The silver in the samples was analysed by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and single particle inductively coupled plasma mass spectrometry (SP-ICP-MS). Microwave digestion was adopted to digest the chitosan antibacterial gel, and then the content of silver was determined by flame atomic absorption spectrometry.
The analysises showed that the particle size of silver in chitosan antibacterial gel was about 150~ 200 nm. The silver showed good linearity in the concentration range of 25~250 μg/L (=0.000 35+0.001 7, =0.999 9). The recovery rate (=9) was 98.5%.
SEM, EDS and SP-ICP-MS can be used for the characterization of silver particles in chitosan antibacterial gel. Microwave digestion-flame atomic absorption spectrophotometry method is simple, practicable, high precision and high quantitative accuracy, which is suitable for the quantitative analysis of silver in chitosan antibacterial gel.
对壳聚糖抗菌凝胶中的银进行表征，并建立样品中银含量的测定方法。
采用扫描电子显微镜（SEM）、能谱仪（EDS）和单颗粒电感耦合等离子体质谱法（SP-ICP-MS）对样品中的银进行分析。采用微波消解对壳聚糖抗菌凝胶进行消解，然后用火焰原子吸收光谱法测定银含量。
分析表明，壳聚糖抗菌凝胶中银的粒径约为150200nm。银在25250μg/L浓度范围内线性良好（=0.000 35+0.001 7，=0.999 9）。回收率（=9）为98.5%。
SEM、EDS和SP-ICP-MS可用于壳聚糖抗菌凝胶中银颗粒的表征。微波消解-火焰原子吸收分光光度法简便、可行、精密度高、定量准确，适用于壳聚糖抗菌凝胶中银的定量分析。
