Serra M T, Castresana M C, Tejerina G
J Basic Microbiol. 1987;27(3):147-53. doi: 10.1002/jobm.3620270306.
Extracellular components of plant pathogenic bacteria were obtained from their culture medium as well as from the whole cells by using NaCl 1 M, pH 6.0; 20% sucrose dissolved in 0.03 M Tris buffer, pH 8.0; or 0.05 M Na2EDTA. All the extracts from Erwinia carotovora subsp. carotovora, Xanthomonas campestris pv. campestris, Pseudomonas syringae pv. phaseolicola, Xanthomonas campestris pv. phaseoli, Pseudomonas solanacearum, and Erwinia carotovora subsp. atroseptica, were assayed for hemagglutinating activity on sheep, rabbit and chicken red blood cells (RBCs). The only active extracts were those obtained by NaCl treatment. They agglutinated sheep and rabbit erythrocytes. Extracts from E. carotovora subsp. atroseptica gave rise to the high agglutination titer on rabbit RBCs. These extracts had the lowest polysaccharide/protein ratio. E. carotovora subsp. carotovora extracts showed only a low titer (18.5 units). The agglutinating activity present in NaCl extracts of the bacteria tested was inhibited by different carbohydrates to various extent. Extracts from E. carotovora subsp. atroseptica appeared to be the most sensitive ones while those of E. carotovora subsp. carotovora least sensitive to the presence of sugar. It is suggested that hemagglutinins observed in plant pathogenic bacteria and those in plant host are similar and that both may, in some way, be involved in the plant-parasite relationship.
通过使用1M、pH 6.0的NaCl；溶解于0.03M、pH 8.0的Tris缓冲液中的20%蔗糖；或0.05M的Na2EDTA，从植物致病细菌的培养基以及全细胞中获取细胞外成分。对胡萝卜软腐欧文氏菌胡萝卜软腐亚种、野油菜黄单胞菌野油菜致病变种、菜豆假单胞菌菜豆致病变种、菜豆黄单胞菌菜豆致病变种、青枯假单胞菌以及黑腐欧文氏菌黑腐亚种的所有提取物，进行了对绵羊、兔子和鸡红细胞（RBCs）的血凝活性测定。唯一有活性的提取物是通过NaCl处理获得的那些。它们能凝集绵羊和兔子的红细胞。黑腐欧文氏菌黑腐亚种的提取物在兔子红细胞上产生了高凝集效价。这些提取物的多糖/蛋白质比率最低。胡萝卜软腐欧文氏菌胡萝卜软腐亚种的提取物仅显示出低凝集效价（18.5个单位）。所测试细菌的NaCl提取物中的凝集活性受到不同碳水化合物的不同程度抑制。黑腐欧文氏菌黑腐亚种的提取物似乎是最敏感的，而胡萝卜软腐欧文氏菌胡萝卜软腐亚种的提取物对糖的存在最不敏感。有人提出，在植物致病细菌中观察到的血凝素与植物宿主中的血凝素相似，并且两者可能在某种程度上参与了植物与寄生物的关系。
