Rote N S, Harrison M R, Scott J R
J Reprod Immunol. 1987 Apr;10(4):273-84. doi: 10.1016/0165-0378(87)90030-1.
IgG and IgM have been identified on the surface of maternal platelets in both autoimmune thrombocytopenia (ATP) and pregnancy-induced hypertension (PIH). IgG is also found on the umbilical cord platelets of patients with ATP and PIH, whereas IgM is only found on the umbilical cord platelets of patients with PIH. The possible maternal or fetal origins of these umbilical cord blood immunoglobulins were investigated by immunoblot analysis of antibodies in paired maternal and umbilical cord blood sera of ATP and PIH patients. Maternal sera contained IgG and IgM antibodies which reacted with several platelet proteins, however, a large amount of patient-to-patient variation was observed in the specific antigens that were identified. Analysis of paired maternal and umbilical cord sera from patients with ATP or PIH showed identical patterns of antigen specificity, which suggested that the IgG antibodies in the fetal circulation were of maternal origin. Circulating IgM antibodies were not observed in the umbilical cord sera of ATP patients. The umbilical cord sera of PIH patients, however, contained IgM antibodies that reacted against a variety of platelet antigens. In addition, most umbilical cord sera from PIH patients had identical patterns and relative intensities of reactivity, which differed from the patterns observed in the paired maternal sera. Antiplatelet IgM in the umbilical cord blood of PIH patients, therefore, appears to be a product of the fetal immune system.
在自身免疫性血小板减少症（ATP）和妊娠高血压综合征（PIH）患者的母体血小板表面均已鉴定出IgG和IgM。在ATP和PIH患者的脐带血小板中也发现了IgG，而IgM仅在PIH患者的脐带血小板中发现。通过对ATP和PIH患者的配对母血和脐带血血清中的抗体进行免疫印迹分析，研究了这些脐带血免疫球蛋白可能的母体或胎儿来源。母血血清中含有与几种血小板蛋白发生反应的IgG和IgM抗体，然而，在所鉴定的特异性抗原中观察到大量患者间的差异。对ATP或PIH患者的配对母血和脐带血血清分析显示出相同的抗原特异性模式，这表明胎儿循环中的IgG抗体来源于母体。在ATP患者的脐带血清中未观察到循环IgM抗体。然而，PIH患者的脐带血清中含有与多种血小板抗原发生反应的IgM抗体。此外，大多数PIH患者的脐带血清具有相同的反应模式和相对反应强度，这与配对母血血清中观察到的模式不同。因此，PIH患者脐带血中的抗血小板IgM似乎是胎儿免疫系统的产物。
