Department of Anesthesiology, Second Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Department of Urology, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.
Sci Rep. 2022 Oct 18;12(1):17064. doi: 10.1038/s41598-022-20775-7.
Prostate cancer (PCa) is a common malignant disease among men and biochemical recurrence (BCR) is considered to be a decisive risk factor for clinical recurrence and PCa metastasis. Clarifying the genes related to BCR and its possible pathways is vital for providing diagnosis and treatment methods to delay the progress of BCR. An analysis of data concerning PCa from previous datasets of The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) was performed. Immunohistochemical (IHC) staining were used to evaluate the expression of SLC14A1 in prostate tissues. Kaplan-Meier analysis, Pearson correlation, and single sample Gene Set Enrichment Analysis (ssGSEA) were used to identify the potential pathway and molecular mechanism of the function of SLC14A1 in BCR of PCa. The expression of SLC14A1 is significantly reduced in prostate cancer cells and tissue comparing to normal prostate epithelial cell and para-cancerous tissue. As indicated by Kaplan-Meier analysis, High expression of SLC14A1 could increase the BCR-free survival time of PCa patients. This effect might be related to the interaction with miRNAs (has-miR-508, has-mir-514a2, and has-mir-449a) and the infiltration of B cells. SLC14A1 is a novel important gene associated with BCR of PCa, and further studies of its molecular mechanism may delay the progress of BCR.
前列腺癌（PCa）是男性常见的恶性疾病，生化复发（BCR）被认为是临床复发和 PCa 转移的决定性危险因素。阐明与 BCR 相关的基因及其可能的途径对于提供诊断和治疗方法以延缓 BCR 的进展至关重要。对来自癌症基因组图谱（TCGA）和基因表达综合数据库（GEO）的先前 PCa 数据集的数据进行了分析。使用免疫组织化学（IHC）染色来评估前列腺组织中 SLC14A1 的表达。采用 Kaplan-Meier 分析、Pearson 相关性和单样本基因集富集分析（ssGSEA）来鉴定 SLC14A1 在 PCa BCR 中的潜在通路和分子机制的功能。与正常前列腺上皮细胞和癌旁组织相比，SLC14A1 在前列腺癌细胞和组织中的表达明显降低。Kaplan-Meier 分析表明，SLC14A1 的高表达可以增加 PCa 患者的 BCR 无复发生存时间。这种作用可能与 miRNA（has-miR-508、has-mir-514a2 和 has-mir-449a）的相互作用和 B 细胞的浸润有关。SLC14A1 是与 PCa BCR 相关的一个新的重要基因，对其分子机制的进一步研究可能会延缓 BCR 的进展。
