Li Xueqian, Qi Jiaqian, Song Xiaofei, Xu Xiaoyan, Pan Tingting, Wang Hong, Yang Jingyi, Han Yue
National Clinical Research Center for Hematologic Diseases, Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, Jiangsu, People's Republic of China.
Institute of Blood and Marrow Transplantation, Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China.
Exp Hematol Oncol. 2022 Oct 18;11(1):74. doi: 10.1186/s40164-022-00335-5.
Acute myeloid leukemia (AML) is a complex, heterogeneous malignant hematologic disease. Although multiple prognostic-related genes gave been explored in previous studies, there are still many genes whose prognostic value remains unclear. In this study, a total of 1532 AML patients from three GEO databases were included, five genes with potential prognostic value (DLC1, NF1B, DENND5B, TANC2 and ELAVL4) were screened by weighted gene co-expression network analysis (WGCNA), least absolute shrinkage and selection operator (LASSO) and support vector machine recursive feature elimination (SVM-RFE). Based on this, we conducted survival analysis of the above five genes through the TCGA database and found that low level of DLC1 was detrimental to the long-term prognosis of AML patients. We also performed external validation in 48 AML patients from our medical center to analyze the impact of DLC1 level on prognosis. In conclusion, DLC1 may be a potential marker affecting the prognosis of AML, and its deficiency is associated with poor prognosis.
急性髓系白血病（AML）是一种复杂的异质性恶性血液病。尽管先前的研究已经探索了多个与预后相关的基因，但仍有许多基因的预后价值尚不清楚。本研究纳入了来自三个基因表达综合数据库（GEO）的1532例AML患者，通过加权基因共表达网络分析（WGCNA）、最小绝对收缩和选择算子（LASSO）以及支持向量机递归特征消除（SVM-RFE）筛选出五个具有潜在预后价值的基因（DLC1、NF1B、DENND5B、TANC2和ELAVL4）。在此基础上，我们通过癌症基因组图谱（TCGA）数据库对上述五个基因进行了生存分析，发现DLC1低表达对AML患者的长期预后不利。我们还在本医学中心的48例AML患者中进行了外部验证，以分析DLC1水平对预后的影响。总之，DLC1可能是影响AML预后的一个潜在标志物，其缺陷与不良预后相关。
