Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Avenida Do Café S/N, Campus da USP, Ribeirão Preto, SP, 14040-904, Brazil.
Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro, Cidade Universitária, Rua Prof. Rodolpho Paulo Rocco 325, Rio de Janeiro, RJ, 21941-617, Brazil.
Clin Oral Investig. 2023 Jan;27(1):345-352. doi: 10.1007/s00784-022-04738-w. Epub 2022 Oct 19.
To assess the influence of estrogen deficiency on tooth eruption rate (TER) and gene expression of estrogen receptor alpha and beta (ERα and ERβ) in the odontogenic region of teeth with continuous formation in a rat model.
Ovariectomies (OVX; n = 25) and sham surgeries (SHAM; n = 25) were performed in female Wistar rats when animals were 25 days old. The TER of the lower incisors, both in impeded (hyperfunction condition) and unimpeded (trimmed incisal edge-hypofunction condition) conditions, was evaluated using standardized digital photographs acquired every 48-72 h for 3 weeks (35th-53rd day of life), using a camera coupled to a stereomicroscope. Quantitative real-time PCR was performed to evaluate the relative gene expression of ERα and ERβ in the odontogenic region.
The OVX group showed a significant reduction in TER when compared to the SHAM group, only in the impeded condition (p = 0.03). There was no statistically significant difference between the groups in ERα gene expression (p = 0.33). ERβ showed a significantly higher gene expression in the OVX group (p ≤ 0.05).
Estrogen deficiency decreases TER in teeth under impeded condition. Estrogen deficiency also increases ERβ gene expression in the odontogenic region of teeth with continuous formation.
Hormonal disturbances affecting estrogen levels can cause alterations in dental formation and teeth eruption.
评估雌激素缺乏对牙齿萌出率（TER）的影响，以及在牙齿持续形成的牙源性区域中雌激素受体α和β（ERα和 ERβ）的基因表达。
在 25 天大的雌性 Wistar 大鼠中进行卵巢切除术（OVX；n=25）和假手术（SHAM；n=25）。使用标准化的数字照片，每隔 48-72 小时拍摄一次，在 3 周（生命第 35-53 天）内评估下切牙的 TER，使用连接到立体显微镜的相机。通过定量实时 PCR 评估牙源性区域中 ERα 和 ERβ的相对基因表达。
与 SHAM 组相比，OVX 组在受限条件下（p=0.03）TER 显著降低。ERα 基因表达在两组之间无统计学差异（p=0.33）。在 OVX 组中，ERβ 基因表达显著升高（p≤0.05）。
雌激素缺乏会降低受限条件下牙齿的 TER。雌激素缺乏还会增加牙齿持续形成的牙源性区域中 ERβ 的基因表达。
影响雌激素水平的激素紊乱可导致牙齿形成和萌出发生改变。
