Department of Genetics and Animal Breeding, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Zakir Hossain Road, Khulshi, Chattogram, 4225, Bangladesh.
Department of Microbiology and Veterinary Public Health, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Zakir Hossain Road, Khulshi, Chattogram, 4225, Bangladesh.
Trop Anim Health Prod. 2022 Oct 19;54(6):350. doi: 10.1007/s11250-022-03347-9.
Polymorphisms in growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) genes have been found to be associated with litter size in goats across the globe. Our previous study detected single-nucleotide polymorphisms (SNPs) in GDF9 and BMP15 genes associated with litter size in Black Bengal, Bangladesh's primary native goat breed. However, Jamunapari and crossbred goats in Bangladesh are yet to be investigated for litter size-associated polymorphisms. In this study, we screened Jamunapari and crossbred (50% Black Bengal × 50% Jamunapari) goats to identify polymorphisms in the GDF9 and BMP15 genes and to assess the association between identified SNPs and litter size. The genomic DNA from 100 female goats (50 Jamunapari and 50 crossbred) was used in polymerase chain reactions (PCRs) to amplify exon 2 of the GDF9 and exon 2 of the BMP15 genes. PCR products were sequenced employing the BigDye Terminator cycle sequencing protocol to identify SNPs. We used a generalized linear model to perform the association analysis for identified SNPs and litter size. Seven SNPs were identified, of which four, C818CT, G1073A, G1189A, and G1330T, were in the GDF9 gene and three, G616T, G735A, and G811A, were in the BMP15 gene. G735A was a synonymous SNP, whereas the remaining were non-synonymous SNPs. Identified SNP loci in GDF9 were low polymorphic (PIC < 0.25), while loci in BMP15 were moderately polymorphic (PIC ≥ 0.25). The genotypes at the G1330T locus had a significant (p < 0.05) difference in litter size in Jamunapari goats, but no significant difference was observed for all genotypes at other loci. Therefore, the G1330T loci could be useful as a marker in marker-assisted selection for litter size traits in goats of Bangladesh.
生长分化因子 9（GDF9）和骨形态发生蛋白 15（BMP15）基因中的多态性已被发现与全球各地的山羊产仔数有关。我们之前的研究在孟加拉国主要的本地山羊品种黑孟加拉羊中检测到与产仔数相关的 GDF9 和 BMP15 基因中的单核苷酸多态性（SNP）。然而，孟加拉国的 Jamunapari 和杂交山羊的产仔数相关多态性尚未得到研究。在这项研究中，我们筛选了 Jamunapari 和杂交（50%黑孟加拉 × 50%Jamunapari）山羊，以鉴定 GDF9 和 BMP15 基因中的多态性，并评估鉴定出的 SNP 与产仔数之间的关联。使用聚合酶链反应（PCR）从 100 只雌性山羊（50 只 Jamunapari 和 50 只杂交）的基因组 DNA 中扩增 GDF9 的外显子 2 和 BMP15 的外显子 2。使用 BigDye Terminator 循环测序方案对 PCR 产物进行测序，以鉴定 SNP。我们使用广义线性模型对鉴定出的 SNP 与产仔数进行关联分析。共鉴定出 7 个 SNP，其中 4 个 SNP，即 C818CT、G1073A、G1189A 和 G1330T，位于 GDF9 基因中，3 个 SNP，即 G616T、G735A 和 G811A，位于 BMP15 基因中。G735A 是同义 SNP，而其余 SNP 是非同义 SNP。GDF9 中的鉴定 SNP 座位低多态性（PIC < 0.25），而 BMP15 中的 SNP 座位中多态性适中（PIC ≥ 0.25）。在 Jamunapari 山羊中，G1330T 座位的基因型在产仔数上有显著差异（p < 0.05），但其他座位的所有基因型均无显著差异。因此，G1330T 座位可作为孟加拉国山羊产仔数性状辅助选择的有用标记。
