Institute of Animal Science, Tibet Academy of Agricultural and Animal Husbandry Science, Lhasa 850009, China.
Isotope Research Laboratory, Sichuan Agricultural University, Ya'an 625014, China.
Genes (Basel). 2023 May 18;14(5):1102. doi: 10.3390/genes14051102.
The Tibetan cashmere goat is a prolific goat breed in China. In sheep breeds, natural mutations have demonstrated that the transforming growth factor beta (TGF-β) super family ligands, such as growth differentiation factor 9 (), bone morphogenetic protein 15 () and their type I receptor (bone morphogenetic protein receptor (), are essential for ovulation and increasing litter size. In this study, 216 female Tibetan cashmere goats were sampled, and candidate genes with fecundity traits were detected via restriction fragment length polymorphism (RFLP) and sequenced. Four polymorphic loci were found in specific amplification fragments of and . Two SNP sites of the gene were discovered, namely G732A and C805G. The G732A mutation did not cause the change in amino acids, and the frequencies of each genotype were 0.695 for the GG type, 0.282 for the GA type and 0.023 for the AA type. The C805G mutation caused amino acids to change from glutamine to glutamate. The genotype frequencies were 0.620 for the CC type, 0.320 for the CG type and 0.320 for the CG type. For the GG type 0.060, the G3 and G4 mutations of the gene were all homozygous mutations. Two known SNP sites, C719T and G1189A, were detected in the Tibetan cashmere goat gene, of which the C719T mutation caused a change of alanine to valine, with a genotype frequency of 0.944 for the CC type and 0.056 for the CT type, whereas no TT type was found. The G1189A mutation caused valine to become isoleucine, and the frequencies of each genotype were 0.579 for the GG type, 0.305 for the GA type and 0.116 for the AA type; G1, B2, B3, B4, FecX, FecX, FecX, G2, G5, G6, G7, G8, FecG, FecTT and FecB mutations were not found in Tibetan cashmere goats. The results of this study provide a data basis for future studies of , and gene mutations in goats.
藏羊是中国产绒量较高的绵羊品种。在绵羊品种中，自然突变表明转化生长因子-β（TGF-β）超家族配体，如生长分化因子 9（）、骨形态发生蛋白 15（）及其 I 型受体（骨形态发生蛋白受体（），对于排卵和增加产仔数是必不可少的。本研究采集了 216 只雌性藏羊样本，通过限制性片段长度多态性（RFLP）和测序检测与繁殖力性状相关的候选基因。在 和 基因的特定扩增片段中发现了 4 个多态性位点。在 基因中发现了 2 个 SNP 位点，即 G732A 和 C805G。G732A 突变未引起氨基酸改变，各基因型的频率分别为 GG 型 0.695、GA 型 0.282 和 AA 型 0.023。C805G 突变导致谷氨酸变为谷氨酸。基因型频率分别为 CC 型 0.620、CG 型 0.320 和 GG 型 0.320。对于 GG 型 0.060， 基因的 G3 和 G4 突变均为纯合突变。在藏羊 基因中检测到 2 个已知的 SNP 位点 C719T 和 G1189A，其中 C719T 突变导致丙氨酸变为缬氨酸，CC 型的基因型频率为 0.944，CT 型为 0.056，未发现 TT 型。G1189A 突变导致缬氨酸变为异亮氨酸，各基因型的频率分别为 GG 型 0.579、GA 型 0.305 和 AA 型 0.116；在藏羊中未发现 G1、B2、B3、B4、FecX、FecX、FecX、G2、G5、G6、G7、G8、FecG、FecTT 和 FecB 突变。本研究结果为今后研究山羊 、 和 基因突变提供了数据基础。
