HIV-1感染的合并或未合并结核共感染患者中Toll样受体（TLR2、TLR4和TLR9）的信使核糖核酸表达
Messenger RNA expression of toll-like receptors (TLR2, TLR4, and TLR9) in HIV-1 infected patients with and without tuberculosis co-infection.
作者信息
Kaushik Gaurav, Vashishtha Richa
机构信息
Department of Allied Health Sciences, School of Allied Health Sciences, Sharda University, Greater Noida, Uttar Pradesh; Department of Medicine, All India Institute of Medical Sciences, New Delhi, India.
Department of Medicine, All India Institute of Medical Sciences, New Delhi, India.
出版信息
Int J Mycobacteriol. 2022 Jul-Sep;11(3):293-298. doi: 10.4103/ijmy.ijmy_108_22.
BACKGROUND
Gene expression levels of TLRs (TLR2, TLR4 and TLR9) are directly involved in the virus recognition and initiation of innate immune responses, therefore, the effect of HIV infection on TLRs gene expression was investigated in functional context through mRNA levels estimations of selected TLRs.
METHODS
In the present study mRNA gene expression of TLR2, TLR4 and TLR9 has been investigated in HIV+ and HIV+TB patients and compared with healthy subjects.
RESULT
The increase expression of TLR2, TLR4 and TLR9 (mRNA level) relative to the internal gene GAPDH was observed in HIV+ and HIV+TB patients as compared to healthy subjects. Similarly, increase in TLRs mRNA expression was observed in HIV+TB patients as compared to HIV+ patients.
CONCLUSION
A modest increase in expression of TLRs in HIV+ patients with and without TB co-infection suggest a potential role for these TLRs in HIV-1 immunopathogenesis.
背景
Toll样受体（TLR2、TLR4和TLR9）的基因表达水平直接参与病毒识别和先天免疫反应的启动，因此，通过对选定Toll样受体的mRNA水平进行评估，在功能背景下研究了HIV感染对Toll样受体基因表达的影响。
方法
在本研究中，对HIV阳性和HIV合并结核病患者的TLR2、TLR4和TLR9的mRNA基因表达进行了研究，并与健康受试者进行了比较。
结果
与健康受试者相比，在HIV阳性和HIV合并结核病患者中观察到相对于内参基因GAPDH，TLR2、TLR4和TLR9（mRNA水平）的表达增加。同样，与HIV阳性患者相比，在HIV合并结核病患者中观察到Toll样受体mRNA表达增加。
结论
在合并或未合并结核病的HIV阳性患者中，Toll样受体表达适度增加表明这些Toll样受体在HIV-1免疫发病机制中具有潜在作用。
Zotero 插件