Takahashi M, Nishina M, Hori E, Matsushita K, Kato K, Ohsaka A
Jpn J Med Sci Biol. 1987 Feb;40(1):27-33. doi: 10.7883/yoken1952.40.27.
1H- and 13C-nuclear magnetic resonance (NMR) spectroscopy was used to study aerobic glucose metabolism in eggs of Angiostrongylus cantonensis in an NCTC-109 medium supplemented with fetal calf serum. Without any pretreatment of the spent medium, we were able to identify and quantitate, by NMR, the end-products of glucose metabolism in eggs after cultivation for 2, 4, and 8 days. We demonstrated that A. cantonensis eggs took up glucose rapidly; among the major end products were found lactic acid, acetic acid and alanine. The eggs are parasitic in a sense that the energy metabolism in them is dependent mainly upon the energy source present in outer medium.
利用1H和13C核磁共振（NMR）光谱法，在添加胎牛血清的NCTC - 109培养基中研究广州管圆线虫虫卵的有氧葡萄糖代谢。在未对用过的培养基进行任何预处理的情况下，我们能够通过NMR鉴定和定量培养2天、4天和8天后虫卵中葡萄糖代谢的终产物。我们证明广州管圆线虫虫卵迅速摄取葡萄糖；主要终产物中发现有乳酸、乙酸和丙氨酸。从某种意义上说，这些虫卵具有寄生性，因为它们的能量代谢主要依赖于外部培养基中存在的能量来源。
