体外培养的成年期广州管圆线虫代谢产物的1H-和13C-核磁共振波谱研究
1H- and 13C-NMR spectroscopic study of the metabolites in young adult Angiostrongylus cantonensis maintained in vitro.
作者信息
Nishina M, Hori E, Matsushita K, Takahashi M, Kato K, Ohsaka A
机构信息
Department of Parasitology, Saitama Medical School, Japan.
出版信息
Mol Biochem Parasitol. 1988 Apr;28(3):249-55. doi: 10.1016/0166-6851(88)90009-6.
1H- and 13C-nuclear magnetic resonance (NMR) spectroscopy were used to study metabolites excreted by young adult Angiostrongylus cantonensis maintained aerobically in the presence of D-[13C6]glucose. End-products of glucose metabolism identified and quantitated by means of 1H-NMR were lactate, acetate and alanine, in the molar ratio of 1:0.13:0.05 for males and 1:0.07:0.04 for females. 13C-NMR analyses proved that all the three products originated from the glucose present in the medium.
利用氢-1（1H）和碳-13（13C）核磁共振（NMR）光谱技术，研究了在含有D-[13C6]葡萄糖的有氧条件下，成年广州管圆线虫幼虫排泄的代谢产物。通过1H-NMR鉴定并定量的葡萄糖代谢终产物为乳酸、乙酸盐和丙氨酸，雄性的摩尔比为1:0.13:0.05，雌性为1:0.07:0.04。13C-NMR分析证明，这三种产物均来源于培养基中的葡萄糖。
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