Yirgu Mihret, Kebede Mulugeta, Feyissa Tileye, Lakew Berhane, Woldeyohannes Aemiro Bezabih
Department of Applied Biology, School of Applied Natural Science, Adama Science and Technology University, P.O. Box 1888, Adama, Ethiopia.
Department of Plant Science, College of Agriculture and Natural Resource, Madda Walabu University, P.O. Box 247, Robe, Oromia, Ethiopia.
Heliyon. 2022 Oct 8;8(10):e10949. doi: 10.1016/j.heliyon.2022.e10949. eCollection 2022 Oct.
Ethiopian barley germplasm is a potential source of useful traits to fight the production challenges of barley farming and to enhance yield productivity in favorable and marginal environments. A study was carried out to assess the distribution and patterns of 17 qualitative trait variations among 85 Ethiopian barley accessions using an alpha lattice design with two replications. The Shannon-Weaver diversity (H') index was used to estimate morphological diversity. Fifteen morphological traits of barley accessions originating from various regions of origins and altitude ranges were polymorphic. However, two traits including stem branching and lemma awn were monomorphic. The highest (0.94) overall mean of H' was obtained for glume colour, kernel row and kernel shape. The estimated H' ranged from 0.41 to 0.99 across regions, and 0.52 to 0.99 across altitude ranges with an overall mean of 0.76. The analysis of variance of H' showed significant variation for most studied traits. Principal components analysis revealed that eight traits were the major loading on the first two principal components that describe 38.3% of the total morphological variance. Heat map analysis based on morphological traits of barley accessions was also grouped into three distinct clusters. Thus, the present finding confirmed that the Ethiopian barley accessions showed vast morphological variations across the region of origins and altitude ranges. Based on the result, further evaluation is ongoing to exploit specific gene variations through phenotyping and genotyping trait association.
埃塞俄比亚大麦种质是应对大麦种植生产挑战以及在有利和边缘环境中提高产量潜力的有用性状的潜在来源。本研究采用α格子设计(重复两次)对85份埃塞俄比亚大麦种质的17个质量性状变异的分布和模式进行了评估。利用香农 - 韦弗多样性(H')指数估计形态多样性。来自不同起源地区和海拔范围的大麦种质的15个形态性状具有多态性。然而,包括茎分枝和颖壳芒在内的两个性状是单态的。颖壳颜色、粒行数和粒形的H'总体均值最高(0.94)。估计的H'在各地区范围为0.41至0.99,在海拔范围内为0.52至0.99,总体均值为0.76。H'的方差分析表明,大多数研究性状存在显著差异。主成分分析显示,八个性状在前两个主成分上的载荷较大,这两个主成分描述了总形态变异的38.3%。基于大麦种质形态性状的热图分析也被分为三个不同的聚类。因此,本研究结果证实,埃塞俄比亚大麦种质在起源地区和海拔范围内表现出巨大的形态变异。基于该结果,正在通过表型和基因分型性状关联进一步评估,以发掘特定的基因变异。