Division of Microbiology, National Institute of Health Sciences, Kawasaki, Japan.
Otsu City Public Health Center, Otsu, Japan.
Foodborne Pathog Dis. 2022 Oct;19(10):704-712. doi: 10.1089/fpd.2022.0048.
is an emerging pathogen causing foodborne infections with diarrhea, abdominal pain, and fever. has been isolated from various food sources, such as chicken and pork. Although many foodborne outbreaks of have been reported, the causative food has not been identified. It is necessary to develop effective detection methods for Because enrichment procedure as the first step of food test is important for growing pathogens, this study aimed to develop a novel effective enrichment for detection in food. In this study, we investigated the optimal concentration and combination of cefixime and tellurite for supplementing modified EC broth (mEC) to effectively isolate from chicken meat. The results showed that mEC supplemented with 50 μg/L cefixime and 2.5 mg/L tellurite (CT-mEC) inhibited the growth of competitive bacteria in chicken meat but not that of . Therefore, it was indicated that CT-mEC had strong potential to selectively grow In an foodborne outbreak, CT-mEC was evaluated. was successfully isolated from a food sample, a kind of salad, by enrichment with CT-mEC but not buffered peptone water and mEC. In this study, CT-mEC as a selective enrichment broth has been developed to detect in chicken meat. It was demonstrated that the selective enrichment broth was effective for the efficient detection of in food.
是一种新兴的病原体，可导致食源性感染，引起腹泻、腹痛和发热。已从各种食物来源中分离出来，如鸡肉和猪肉。虽然已经报告了许多由引起的食源性疫情，但致病食物尚未确定。有必要开发有效的检测方法，因为富集程序是食品检测的第一步，对于培养病原体非常重要。本研究旨在开发一种新型有效的富集方法，用于检测食品中的。在这项研究中，我们研究了头孢克肟和亚碲酸盐的最佳浓度和组合，以补充改良 EC 肉汤（mEC），从而有效地从鸡肉中分离。结果表明，mEC 中添加 50μg/L 头孢克肟和 2.5mg/L 亚碲酸盐（CT-mEC）抑制了鸡肉中竞争细菌的生长，但不抑制的生长。因此，表明 CT-mEC 具有选择性生长的强大潜力。在一次食源性疫情中，评估了 CT-mEC。通过 CT-mEC 富集而非缓冲蛋白胨水和 mEC，成功地从一种沙拉食品样本中分离出。在本研究中，已经开发出 CT-mEC 作为一种选择性富集肉汤来检测鸡肉中的。结果表明，该选择性富集肉汤可有效用于食品中高效检测。
