Zafar Muhammad Zeeshan, De Rauw Klara, Van den Abeele Anne-Marie, Joossens Marie, Heyvaert Lore, Houf Kurt
Department of Veterinary and Biosciences, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Laboratory of Microbiology, Sint-Lucas Hospital, Groenebriel 1, 9000 Ghent, Belgium.
Microorganisms. 2024 Nov 23;12(12):2408. doi: 10.3390/microorganisms12122408.
The significance of as a foodborne pathogen is increasingly acknowledged, but the assessment of its occurrence and transmission remains challenging due to the lack of validation of selective isolation, detection, and identification methods. The aim of the present study was to examine its presence on various meat samples at the retail level in order to assess a potential foodborne transmission and its occurrence in clinical stool samples. First, the evaluation and selection of a selective enrichment broth and isolation medium, combined with an optimized identification by MALDI-TOF MS, as well as a suitable DNA extraction method and a PCR-based detection strategy were developed. After the evaluation of existing isolation strategies and the formulation of an adapted enrichment and isolation medium, 100% isolation specificity was not achieved. An identity confirmation of suspected colonies remains necessary. A total of 292 samples, including 45 beef fillet, 51 minced beef, 50 pork fillet, 30 minced pork, 30 chicken carcass, 51 chicken fillet, and 35 minced chicken samples were examined. Samples were all collected at the retail level, including supermarkets and local butcheries. was isolated from two chicken fillets (3.9%) and additionally detected in one minced chicken (4.5%) and two other chicken fillet (4.5%) samples by a PCR assay. All beef and pork samples tested negative for its presence, but transmission through these meat types cannot be excluded, as it potentially correlates with the level of fecal contamination that was significantly higher on poultry products. With other hygienic conditions and processing steps applied, the presence of on food can therefore differ in other parts of the world. was present in 0.4% of the 2419 clinical stool samples examined. The future development of a chromogenic isolation medium, as well as further extensive epidemiologic approaches and a genomic comparison of human, food, and animal isolates, could enhance the assessment of the emerging pathogen status and its potential as a foodborne hazard.
作为一种食源性病原体的重要性日益得到认可，但由于缺乏对选择性分离、检测和鉴定方法的验证，对其发生和传播的评估仍然具有挑战性。本研究的目的是检查其在零售层面各种肉类样品中的存在情况，以评估潜在的食源性传播及其在临床粪便样本中的发生情况。首先，开发了选择性富集肉汤和分离培养基的评估与选择方法，结合基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）进行优化鉴定，以及合适的DNA提取方法和基于PCR的检测策略。在评估现有分离策略并配制改良的富集和分离培养基后，未实现100%的分离特异性。对疑似菌落进行身份确认仍然是必要的。共检查了292个样品，包括45个牛里脊、51个牛肉末、50个猪里脊、30个猪肉末、30个鸡 carcass、51个鸡里脊和35个鸡肉末样品。所有样品均在零售层面采集，包括超市和当地肉店。从两个鸡里脊（3.9%）中分离出该病原体，另外通过PCR检测在一个鸡肉末（4.5%）和另外两个鸡里脊（4.5%）样品中检测到。所有牛肉和猪肉样品检测均为阴性，但不能排除通过这些肉类类型传播，因为其潜在与粪便污染水平相关，而家禽产品上的粪便污染水平明显更高。由于应用了其他卫生条件和加工步骤，因此在世界其他地区食品上该病原体的存在情况可能不同。在所检查的2419份临床粪便样本中，该病原体存在于0.4%的样本中。开发显色分离培养基、进一步广泛的流行病学方法以及对人类、食品和动物分离株进行基因组比较，未来可能会加强对这种新兴病原体状况及其作为食源性危害潜力的评估。
