Functional study and epigenetic targets analyses of in intramuscular preadipocytes via ChIP-seq and mRNA-seq.

The epigenetic regulator is involved in hepatic lipid homoeostasis. However, the role of in regulating intramuscular fat deposition as well as the pathways and potential epigenetic targets involved remain unknown. Herein, we investigate function, its genome-wide epigenetic target profile, and transcriptomic changes under overexpression during yak intramuscular preadipocytes differentiation. To this end, we analysed the relationship between and intramuscular fat content as well as lipid metabolism-related genes in tissue. We found that expression negatively correlates with intramuscular fat content as well as with the expression of genes related to lipid synthesis, while positively correlating with that of fatty acid oxidation-involved genes. overexpression in intramuscular preadipocytes significantly reduced adipose differentiation marker expression, intracellular triacylglycerol content, and lipid deposition. Chromatin immunoprecipitation coupled with high-throughput sequencing of H3K4ac (a known direct target of ) and high-throughput mRNA sequencing results revealed that may regulate intramuscular fat deposition via three potential new transcription factors (NRF1, NKX3.1, and EGR1) and four genes (, and ) implicated in protein processing within the endoplasmic reticulum pathway and the MAPK signalling pathway in yaks. Our study provides novel insights into the role of in regulating yak intramuscular fat deposition and may help clarify the mechanistic determinants of yak meat characteristics.