ICAR-Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswer, 751002, India.
ICAR-Central Inland of Fisheries Research Institute, Barrackpore, Kolkata, India.
Fish Physiol Biochem. 2022 Dec;48(6):1427-1442. doi: 10.1007/s10695-022-01133-w. Epub 2022 Oct 20.
Brewer's spent yeast (BSY) is among the most voluminous by-products generated in brewery industry that adds to the waste; however, smart utilization of BSY could lead to edible biomass production besides waste management. To utilize it for biomass production, it is being used in fish feeds; however, its effect on the fish physiology has been scantily studied. The present study investigated the proteomic changes in muscle tissues of carp Labeo rohita fed with BSY-based diet, to understand its impact on muscle physiology and biomass. Six feeds were prepared with different grades of BSY (0, 20, 30, 40, 50, 100% replacement of fishmeal with BSY) and fishes were fed for 90 days. Highest weight gain%, feed conversion efficiency, specific growth rate% were observed in 30% BSY-replaced group and this group was considered for the proteomic study. Comparative shotgun proteomic analysis was carried out by LC-MS/MS and data generated have been deposited in ProteomeXchange Consortium with dataset identifier PXD020093. A total of 62 proteins showed differential abundance; 29 increased and 33 decreased in the 30% BSY-replaced group. Pathway analysis using IPA and Panther tools revealed that the proteins tyrosine protein kinase, PDGFα, PKRCB and Collagen promote muscle growth by inducing the PI3K-AKT pathway. Conversely, the proteins Serine/threonine-protein phosphatase, Phosphatidylinositol 3,4,5-trisphosphate5-phosphatase 2A and Ras-specific guanine- nucleotide-releasing factor inhibit muscle growth indicating that 30% BSY-replaced feed promote muscle growth in a highly controlled manner. Findings suggest that BSY could be recycled for carp feed production in large scale thereby leading to resource conservation, reducing environmental effects.
啤酒废酵母（BSY）是啤酒工业中产生的最大量的副产物之一，增加了废物量；然而，智能利用 BSY 除了废物管理外，还可以生产可食用的生物质。为了将其用于生产生物质，它被用于鱼类饲料中；然而，其对鱼类生理学的影响研究甚少。本研究调查了鲤鱼肌肉组织中基于 BSY 的饮食的蛋白质组变化，以了解其对肌肉生理学和生物质的影响。用不同等级的 BSY（0、20、30、40、50、100%用 BSY 替代鱼粉）制备了六种饲料，并对鱼类进行了 90 天的喂养。在 30% BSY 替代组中观察到最高的体重增加%、饲料转化率%和特定生长率%，该组被认为用于蛋白质组学研究。通过 LC-MS/MS 进行了比较 shotgun 蛋白质组学分析，生成的数据已存入 ProteomeXchange 联合会，数据集标识符为 PXD020093。共有 62 种蛋白质显示出差异丰度；30% BSY 替代组中 29 种增加，33 种减少。IPA 和 Panther 工具的途径分析显示，酪氨酸蛋白激酶、PDGFα、PKRCB 和胶原蛋白等蛋白质通过诱导 PI3K-AKT 途径促进肌肉生长。相反，丝氨酸/苏氨酸蛋白磷酸酶、磷脂酰肌醇 3,4,5-三磷酸 5-磷酸酶 2A 和 Ras 特异性鸟嘌呤核苷酸释放因子等蛋白质抑制肌肉生长，表明 30% BSY 替代饲料以高度受控的方式促进肌肉生长。研究结果表明，BSY 可以在大规模上回收用于鲤鱼饲料生产，从而实现资源节约，减少环境影响。
