Vieira Elsa F, das Neves José, Vitorino Rui, Dias da Silva Diana, Carmo Helena, Ferreira Isabel M P L V O
LAQV/REQUIMTE, Departamento de Ciências Químicas, Laboratório de Bromatologia e Hidrologia, Faculdade de Farmácia, Universidade do Porto , 4050-313, Porto, Portugal.
i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto , 4200-135, Porto, Portugal.
J Agric Food Chem. 2016 Oct 5;64(39):7335-7341. doi: 10.1021/acs.jafc.6b02719. Epub 2016 Sep 20.
Brewer's spent yeast (BSY) autolysates may have potential applications as food ingredients or nutraceuticals due to their antioxidant and ACE-inhibitory activities. The impact of simulated gastrointestinal (GI) digestion, the interaction with intracellular sources of oxidative stress, the intestinal cell permeability of BSY peptides, and the antioxidant and ACE-inhibitory activities of BSY permeates were assayed. Gastrointestinal digestion of BSY autolysates enhanced antioxidant and ACE-inhibitory activities as measured in vitro. No cytotoxic effects were observed on Caco-2 cells after exposure to the digested BSY autolysates within a concentration range of 0.5 to 3.0 mg of peptides/mL. A protective role to induced oxidative stress was observed. The transepithelial transport assays indicate high apparent permeability coefficient (P) values for BSY peptides across Caco-2/HT29-MTX cell monolayer (14.5-26.1 × 10 cm/s) and for Caco-2 cell monolayer model (12.4-20.8 × 10 cm/s), while the antioxidant and ACE-inhibitory activities found in flux material from the basolateral side suggest transepithelial absorption of bioactive compounds.
由于具有抗氧化和血管紧张素转换酶（ACE）抑制活性，啤酒废酵母（BSY）自溶物可能具有作为食品成分或营养保健品的潜在应用。测定了模拟胃肠道（GI）消化的影响、与细胞内氧化应激源的相互作用、BSY肽的肠细胞通透性以及BSY渗透物的抗氧化和ACE抑制活性。体外测定结果表明，BSY自溶物经胃肠道消化后，其抗氧化和ACE抑制活性增强。在浓度范围为0.5至3.0mg肽/mL的消化后的BSY自溶物作用于Caco-2细胞后，未观察到细胞毒性作用。观察到其对诱导的氧化应激具有保护作用。跨上皮转运试验表明，BSY肽穿过Caco-2/HT29-MTX细胞单层（14.5 - 26.1×10 cm/s）和Caco-2细胞单层模型（12.4 - 20.8×10 cm/s）时具有较高的表观渗透系数（P）值，而从基底外侧通量物质中发现的抗氧化和ACE抑制活性表明生物活性化合物可进行跨上皮吸收。
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