Department of Human Genetics, McGill University, Montreal, QC, Canada.
Institute of Parasitology, McGill University, Montreal, QC, Canada.
Methods Mol Biol. 2023;2594:185-204. doi: 10.1007/978-1-0716-2815-7_14.
MicroRNAs exert their effects in the context of gene regulatory networks. The recent development of high-throughput experimental approaches and the growing availability of gene expression data have permitted comprehensive functional studies of miRNAs. However, the data interpretation is often challenging due to the fact that miRNAs not only act cooperatively with other miRNAs but also participate in complex networks by interacting with other functional elements, including non-coding RNAs or transcription factors that often have extensive effects on cell biology. This chapter provides detailed practical procedures on how to use miRNet 2.0 ( https://www.mirnet.ca ) to perform miRNA regulatory network analytics to gain functional insights.
MicroRNAs 在基因调控网络的背景下发挥作用。高通量实验方法的最新发展和基因表达数据的不断增加，使得对 miRNAs 的全面功能研究成为可能。然而，由于 miRNA 不仅与其他 miRNA 合作发挥作用，而且通过与其他功能元件（包括非编码 RNA 或转录因子）相互作用参与复杂网络，这些功能元件通常对细胞生物学有广泛的影响，因此数据解释常常具有挑战性。本章提供了详细的实用程序，介绍如何使用 miRNet 2.0（https://www.mirnet.ca）来执行 miRNA 调控网络分析，以获得功能见解。
