Laboratory of Medicinal and Aromatic Plants, Centre of Biotechnology of Borj Cedria, Hammam-Lif, Tunisia.
Int J Environ Health Res. 2024 Jan;34(1):191-200. doi: 10.1080/09603123.2022.2137475. Epub 2022 Oct 20.
The methanolic extracts of cypress ( L.) collected at three phenological stages were evaluated for their cytotoxicity on Vero cells by MTT test as well as on (HSV-2) and coxsackie (CVB-3) viruses by plaque reduction assay. The methanolic extract exhibited the highest cytotoxicity against HSV-2 (IC = 20.40 µg/mL) and CVB-3 (IC = 47.50 µg/mL) at the flowering stage. This extract also exhibited a virucidal action both during the entry of viruses and the release of newly formed virions. The methanolic extract bioguided purification showed that the ethyl-acetate fraction was responsible for virucidal activity. This fraction was endowed with more important selectivity index of 8.15 for HSV-2 and 4.40 for CVB-3. The ethyl acetate fraction was subjected to thin layer chromatography fractionation and identification by HPLC-DAD-ESI-MS. Results showed that the condensed tannin procyanidin B2 was identified for the first time responsible of the antiviral activity of cypress.
从三种物候期采集的柏木（Cupressus ）甲醇提取物，通过 MTT 试验评价其对 Vero 细胞的细胞毒性，以及通过蚀斑减少试验评价对单纯疱疹病毒 2 型（HSV-2）和柯萨奇病毒 B3 型（CVB-3）的抗病毒活性。在开花期，甲醇提取物对 HSV-2（IC = 20.40 µg/mL）和 CVB-3（IC = 47.50 µg/mL）的细胞毒性最高。该提取物在病毒进入和新形成的病毒释放过程中均表现出病毒灭活作用。甲醇提取物的生物导向纯化表明，乙酸乙酯部分具有病毒灭活活性。该部分对 HSV-2 和 CVB-3 的选择性指数分别为 8.15 和 4.40。乙酸乙酯部分进行薄层色谱分离，并通过 HPLC-DAD-ESI-MS 进行鉴定。结果表明，首次鉴定出缩合单宁原花青素 B2 对柏木的抗病毒活性负责。
