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侵染中国黄瓜的西瓜尖镰孢菌4号生理小种全基因组测序

Whole-Genome Sequencing of f. sp. Strain Race-4 Infecting Cucumber in China.

作者信息

Yang Jingjing, Mao Aijun, Zhang Jian, Zhang Xiaofei, Xia Changxuan, Zhao Hong, Wang Yixin, Wen Changlong, Liu Hui, Wang Qian

机构信息

Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, China.

Beijing Vegetable Research Center (BVRC), Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China.

出版信息

Plant Dis. 2023 Apr;107(4):1210-1213. doi: 10.1094/PDIS-08-22-1815-A. Epub 2023 Apr 19.

Abstract

f. sp. , which causes root and vascular wilting, is one of the most devastating diseases infecting cucumber. Here, we report the first genome resource with high-quality assembly for f. sp. strain Race-4, which is primarily endemic to China. The genome was 59.11 Mb in size and consisted of 48 scaffolds with an N50 of 3.87 Mb using PacBio long reads (301.77×) sequencing, and encodes 14,898 proteins from analyzing RNA-seq data. Gene annotations identified pathogen-host interaction genes, fungal virulence factors, secreted proteins, transcription factors, and secondary metabolite biosynthesis gene. Moreover, functional genes reported in previous studies were also identified in the genome of Race-4. These genes and genome resource may play important roles in understanding f. sp. -cucumber interactions and will be useful for further research.

摘要

引起黄瓜根和维管束萎蔫的[黄瓜枯萎病菌]黄瓜专化型,是侵染黄瓜最具毁灭性的病害之一。在此,我们报道了首个针对主要在中国流行的黄瓜枯萎病菌黄瓜专化型小种4菌株的高质量组装基因组资源。该基因组大小为59.11 Mb,使用PacBio长读长(301.77×)测序由48个支架组成,N50为3.87 Mb,通过分析RNA-seq数据编码14,898个蛋白质。基因注释鉴定出病原体-宿主相互作用基因、真菌毒力因子、分泌蛋白、转录因子和次生代谢物生物合成基因。此外,在小种4的基因组中也鉴定出了先前研究报道的功能基因。这些基因和基因组资源可能在理解黄瓜枯萎病菌与黄瓜的相互作用中发挥重要作用,并将有助于进一步的研究。

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