Cao Xiulan, Ye Yuting, Li Peng
Key Laboratory of Ministry of Education for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, Hainan Normal University, Haikou 571158, China.
Plant Dis. 2023 Mar;107(3):919-921. doi: 10.1094/PDIS-06-22-1339-A. Epub 2023 Jan 14.
HNU47 was originally isolated from rhizosphere soil of stock in a continuous cropping tomato-planting field, which has excellent antagonistic ability against . Here, we sequenced the genome of HNU47 to gain insights into the underlying basis of its antagonistic activity. Results of phylogenetic analysis of the whole genomic sequence demonstrated that HNU47 belongs to . Through antiSMASH analysis, 10 secondary metabolite biosynthesis gene clusters were predicted. There were only two gene clusters with similarity higher than 95% with known compounds' gene clusters and the similarities of the other eight gene clusters were lower than 30%, including three gene clusters with no homology. In addition, biocontrol experiments confirmed that HNU47 could decrease the incidence of bacterial wilt caused by on tomato. These findings support the potential of developing HNU47 as a biocontrol agent against by producing some unknown active compounds. The genome sequence reported here is also useful for revealing the modulation mechanisms underlying biosynthesis of active compounds.
HNU47最初是从连作番茄种植田的砧木根际土壤中分离出来的，它对……具有出色的拮抗能力。在此，我们对HNU47的基因组进行了测序，以深入了解其拮抗活性的潜在基础。全基因组序列的系统发育分析结果表明，HNU47属于……通过antiSMASH分析，预测出10个次级代谢物生物合成基因簇。只有两个基因簇与已知化合物的基因簇相似度高于95%，其他八个基因簇的相似度低于30%，其中包括三个无同源性的基因簇。此外，生物防治实验证实，HNU47可以降低番茄上由……引起的青枯病发病率。这些发现支持了将HNU47开发为一种通过产生一些未知活性化合物来防治……的生物防治剂的潜力。这里报道的基因组序列对于揭示活性化合物生物合成的调控机制也很有用。
