Laboratory of Food Chemistry and Biochemistry & Leuven Food Science and Nutrition Research Centre (LFoRCe), KU Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee, Belgium.
Laboratory of Food Chemistry and Biochemistry & Leuven Food Science and Nutrition Research Centre (LFoRCe), KU Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee, Belgium.
Food Chem. 2023 Mar 15;404(Pt A):134584. doi: 10.1016/j.foodchem.2022.134584. Epub 2022 Oct 13.
Vitamin A is generally analysed using a time-consuming and possibly detrimental saponification step, followed by extraction and HPLC analysis. We here developed a new method to analyse retinyl palmitate (RP) (also known as vitamin A palmitate) without the need for saponification and validated it in model systems consisting of RP, soy oil and wheat bran, and in RP-fortified cereal products. Two direct solvent extraction protocols using acetone/methanol (7/3, v/v) or chloroform/methanol (1/1, v/v) were tested. After extraction, RP was quantified by reversed-phase HPLC with UV detection. The HPLC method had low limits of detection (0.01 µg/mL) and quantification (0.03 µg/mL). Both extraction protocols showed a good recovery (88-105 %) and intra-and inter-day precision (<5%) for RP extraction from the model systems. The obtained results corresponded to results obtained using a golden standard approach. For complex food matrices like bread and cookies, the chloroform/methanol extraction protocol showed the best performance characteristics.
维生素 A 通常采用耗时且可能有害的皂化步骤进行分析，然后进行提取和 HPLC 分析。我们在此开发了一种新的方法，无需皂化即可分析棕榈酸视黄酯 (RP)（也称为维生素 A 棕榈酸酯），并在由 RP、大豆油和麦麸组成的模型系统以及 RP 强化谷物产品中对其进行了验证。使用丙酮/甲醇 (7/3，v/v) 或氯仿/甲醇 (1/1，v/v) 测试了两种直接溶剂提取方案。提取后，通过反相 HPLC 结合 UV 检测定量 RP。HPLC 方法的检测限 (0.01 µg/mL) 和定量限 (0.03 µg/mL) 均较低。对于从模型系统中提取 RP，这两种提取方案均显示出良好的回收率 (88-105%) 和日内和日间精密度 (<5%)。获得的结果与使用黄金标准方法获得的结果相对应。对于面包和饼干等复杂的食物基质，氯仿/甲醇提取方案表现出最佳的性能特征。
