Department of Chemistry, Kyungpook National University, Daegu 41566, Republic of Korea.
Department of Food Science and Biotechnology, Graduate School, Kyungpook National University, Daegu 41566, Republic of Korea; Food and Bio-Industry Research Institute, Inner Beauty/Antiaging Center, Kyungpook National University, Daegu 41566, Republic of Korea.
Food Chem. 2023 Mar 15;404(Pt A):134544. doi: 10.1016/j.foodchem.2022.134544. Epub 2022 Oct 7.
Nymphaea rubra flowers (NRF) are widely used as a food and in folk medicine throughout the subtropical regions due to their health-promoting characteristics. This study characterized the phytochemical composition of various extracts/fractions of NRF by establishing a quadrupole-cyclic ion mobility-time-of-flight (Q-cIM-TOF) mass spectrometry method in both positive and negative electrospray ionization modes. Over 100 phytoconstituents were tentatively identified, among which 53 phytochemicals belonging to phenolic acids, tannins, flavonoids, terpenoids, alkaloids, xanthones, and naphthopyrones have never been documented in NRF before. Moreover, the ethyl acetate fraction of NRF demonstrated strong antioxidant potential (IC: 9.21 ± 0.47 μg/mL in DPPH assay and 13.65 ± 0.03 μg/mL in ABTS assay) and tyrosinase, α-glucosidase, and elastase inhibition (IC: 10.58 ± 0.20, 2.48 ± 0.02, and 38.15 ± 0.25 μg/mL, respectively). The findings highlight the value of NRF as a source of functional components and broaden its potential applications in the food and nutraceutical industries.
红睡莲花朵（NRF）由于其具有促进健康的特性，在亚热带地区被广泛用作食品和民间药物。本研究通过建立四极杆-循环离子淌度-飞行时间（Q-cIM-TOF）质谱法，在正、负离子喷雾模式下，对 NRF 的各种提取物/馏分的植物化学成分进行了表征。初步鉴定了 100 多种植物成分，其中 53 种植物化学物质属于酚酸、单宁、类黄酮、萜类、生物碱、酮和萘并吡喃酮，以前从未在 NRF 中记录过。此外，NRF 的乙酸乙酯馏分表现出很强的抗氧化潜力（在 DPPH 测定中 IC 为 9.21±0.47μg/mL，在 ABTS 测定中 IC 为 13.65±0.03μg/mL），并具有酪氨酸酶、α-葡萄糖苷酶和弹性蛋白酶抑制活性（IC 分别为 10.58±0.20、2.48±0.02 和 38.15±0.25μg/mL）。这些发现强调了 NRF 作为功能性成分来源的价值，并拓宽了其在食品和营养保健品行业的潜在应用。
