Ambarwati Neneng Siti Silfi, Sukma Nurnisya Tiara, Desmiaty Yesi, Auliya Annisa, Budi Setia, Arifuddin M, Ahmad Islamudin
Department of Cosmetology, Engineering Faculty and The Research Center for Cosmetics, Research and Community Services Institute, Universitas Negeri Jakarta, East Jakarta, Indonesia.
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Pancasila, Jagakarsa, South Jakarta, Indonesia.
J Adv Pharm Technol Res. 2024 Jan-Mar;15(1):8-12. doi: 10.4103/japtr.japtr_344_23. Epub 2024 Jan 15.
and King are native plants from Indonesia and have tremendous potential as a source of raw medicines based on local wisdom. However, scientific data for strengthening pharmaceuticals are still limited. Therefore, it is necessary to conduct a study to strengthen and develop the potential of both plants using the approach of traditional medicine. This study aimed to explore the secondary metabolite composition and biological activity (antioxidant and antielastase) of both plants. Both samples were extracted using 70% ethanol and microwave-assisted extraction with a microwave power of 120 watts for 15 min. The extract obtained was then screened for phytochemicals using specific reagents. The total phenolic content (TPC) was determined using spectrophotometry with a 96-well microplate reader method. The total flavonoid content (TFC) was determined using the colorimetric method, whereas metabolite profiling analysis was conducted using the UPLC-QToF-MS/MS system. Meanwhile, biological activity was tested for antioxidant activity and antielastase as measured by a microplate reader 96-well spectrophotometry method at specific wavelengths. According to the results, and fruit peel extracts showed positive detection of particular secondary metabolites. TPC and TFC values were 13.98 ± 1.90 mg GAE/g and 10.33 ± 1.90 mg QE/g for and 11.98 ± 2.04 mgGAE/g and 1.96 ± 0.36 mgQE/g for . Metabolite profiling detected some compounds from , including ephedrannin B, hinokiflavone, mahuannin J, and candidate mass CHO, and , including 5-Hydroxy-7,8,2'- trimethoxyflavone, lucialdehyde B, candidate mass CHNO, candidate mass CHO, and candidate mass CHO. Meanwhile, the biological activities (antioxidant and antielastase) were 137.721 μg/mL and 108.893 μg/mL for and 481.948 μg/mL and 250.611 μg/mL for , respectively. Both plants showed different profiles of secondary metabolites and biological activities (antioxidant and antielastase) according to their respective characteristics.
和金是来自印度尼西亚的本土植物，基于当地智慧，它们作为生药来源具有巨大潜力。然而，用于强化药物的科学数据仍然有限。因此，有必要开展一项研究，采用传统医学方法来强化和开发这两种植物的潜力。本研究旨在探索这两种植物的次生代谢物组成及生物活性（抗氧化和抗弹性蛋白酶）。两种样品均用70%乙醇并采用120瓦微波功率进行15分钟的微波辅助提取。然后用特定试剂对所得提取物进行植物化学物质筛选。总酚含量（TPC）采用96孔酶标仪分光光度法测定。总黄酮含量（TFC）采用比色法测定，而代谢物谱分析则使用超高效液相色谱-四极杆飞行时间串联质谱（UPLC-QToF-MS/MS）系统进行。同时，通过酶标仪96孔分光光度法在特定波长下测定抗氧化活性和抗弹性蛋白酶的生物活性。根据结果，和金果皮提取物对特定次生代谢物检测呈阳性。和金的TPC和TFC值分别为13.98±1.90毫克没食子酸当量/克和10.33±1.90毫克槲皮素当量/克，和金的分别为11.98±2.04毫克没食子酸当量/克和1.96±0.36毫克槲皮素当量/克。代谢物谱分析从和金中检测到一些化合物，包括麻黄宁B、扁柏黄酮、马欢宁J和候选质量CHO，从和金中检测到包括5-羟基-7,8,2'-三甲氧基黄酮、卢西醛B、候选质量CHNO、候选质量CHO和候选质量CHO。同时，和金的生物活性（抗氧化和抗弹性蛋白酶）分别为137.721微克/毫升和108.893微克/毫升，和金的分别为481.948微克/毫升和250.611微克/毫升。这两种植物根据各自特性呈现出不同的次生代谢物谱和生物活性（抗氧化和抗弹性蛋白酶）。
