Department of Zoology, Pachhunga University College, Mizoram University, Aizawl 796001, Mizoram, India.
Department of Zoology, Pachhunga University College, Mizoram University, Aizawl 796001, Mizoram, India.
Parasitol Int. 2023 Feb;92:102690. doi: 10.1016/j.parint.2022.102690. Epub 2022 Oct 17.
Prosorhynchoides aspinosus n. sp., a new species of digenean trematode, is described based on morphological, ultrastructural and molecular data from the freshwater fish, Xenentodon cancila Hamilton, 1822, of Tuikum and Tuirial river in Mizoram, Northeast India. It differs from its congeners in the absence of tegumental spines and the length of cirrus sac. These unique morphological characteristics were further supported by the ultrastructural data showing distinct knob-like protrusions in the tegument. Each knob-like protrusion is without cutting plates or spines and appears granulated at higher magnification. The molecular data analyses, along with the sequences of other Prosorhynchoides available in the Genbank, using internal transcribed spacer 2 and 28S rDNA revealed a gap of 4% K2P (Kimura 2 parameter) distance with its closest congener, P. karvei, confirming the novelty of the species, and thus, clearly differentiates the new species from all other valid Prosorhynchoides spp.
本文基于来自印度东北部米佐拉姆邦图伊库姆和图里亚尔河的淡水鱼 Xenentodon cancila Hamilton, 1822 的形态学、超微结构和分子数据，描述了一种新的 digenean 吸虫 Prosorhynchoides aspinosus n. sp.。它与同属的其他种不同之处在于没有表皮刺和纤毛囊的长度。这些独特的形态特征进一步得到了超微结构数据的支持，显示出表皮上有明显的块状突起。每个块状突起没有切割板或刺，在更高的放大倍数下呈现出颗粒状。分子数据分析，以及 Genbank 中其他可用的 Prosorhynchoides 的序列，使用内部转录间隔区 2 和 28S rDNA 显示与最接近的同属种 P. karvei 的 K2P（Kimura 2 参数）距离有 4%的差距，证实了该物种的新颖性，从而将新种与所有其他有效的 Prosorhynchoides spp. 明显区分开来。
