Department of Microbiology, AHEPA University Hospital, School of Medicine, Aristotle University of Thessaloniki, Greece.
Department of Microbiology, AHEPA University Hospital, School of Medicine, Aristotle University of Thessaloniki, Greece.
J Glob Antimicrob Resist. 2022 Dec;31:252-255. doi: 10.1016/j.jgar.2022.10.008. Epub 2022 Oct 17.
In this communication, we describe the emergence of the mcr-1 colistin resistance gene in a blaCTX-M-32 extended-spectrum-β-lactamase-producing Escherichia coli isolate recovered from a pediatric patient in Greece.
Bacterial identification and antimicrobial susceptibility testing were performed with the VITEK2 automated system and broth microdilution. Detection of resistance genes, assignment to sequence type, in silico plasmid detection, and virulence factors were carried out using ResFinder, MLST 2.0, PlasmidFinder 2.1., and VirulenceFinder 2.0, respectively. PlasmidSPAdes v3.11.1 was used to assemble the plasmid contigs. The mcr-1.1-containing plasmid was analyzed for insertion sequence elements using ISfinder. Phylogenetically relevant sequences of the plasmid were identified using the Microbe BLASTN suite.
The microorganism was assigned to sequence type 48 and carried four plasmids of different incompatibility groups. The specific mcr-1.1 allele was located in a 32.722 bp plasmid belonging to the IncX4 group with no additional resistance genes.
To the best of our knowledge, this is the first detection of mcr-1 in a human specimen in our country. A potential spread of mcr-1 in Greece is concerning because of the existing high rates of carbapenem resistance and colistin usage as a last resort regimen.
在本通讯中，我们描述了 mcr-1 黏菌素耐药基因在一株从希腊儿科患者中分离出的产 blaCTX-M-32 超广谱β-内酰胺酶的大肠埃希菌中的出现。
采用 VITEK2 自动化系统和肉汤微量稀释法进行细菌鉴定和药敏试验。使用 ResFinder、MLST 2.0、PlasmidFinder 2.1 和 VirulenceFinder 2.0 分别检测耐药基因、序列型分类、计算机质粒检测和毒力因子。使用 PlasmidSPAdes v3.11.1 组装质粒的连续体。使用 ISfinder 分析携带 mcr-1.1 的质粒中的插入序列元件。使用 Microbe BLASTN 套件鉴定质粒中与系统发育相关的序列。
该微生物被分配到 48 型序列，并携带四个不同不相容群的质粒。特定的 mcr-1.1 等位基因位于属于 IncX4 组的 32722 bp 质粒中，没有其他耐药基因。
据我们所知，这是在我国首次在人类标本中检测到 mcr-1。由于存在高碳青霉烯类耐药率和使用黏菌素作为最后手段的情况，mcr-1 在希腊的潜在传播令人担忧。
