Thailand-Japan Research Collaboration Center on Emerging and Re-emerging Infections (RCC-ERI), Nonthaburi 11000, Thailand.
National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, Thailand.
J Glob Antimicrob Resist. 2019 Mar;16:168-169. doi: 10.1016/j.jgar.2019.01.009. Epub 2019 Jan 16.
Escherichia coli isolates carrying the mcr-1 gene are rarely reported in diarrhoeal patients. Here we report the draft genome sequence of a colistin-resistant E. coli isolated from a hospitalised patient with acute diarrhoea in Thailand.
Whole genomic DNA of the colistin-resistant E. coli isolate (MSF11) was extracted and was sequenced using an Ion Torrent sequencer with 400-bp read chemistry. The draft genome sequence of MSF11 was analysed with regard to multilocus sequence type (ST), serotype, acquired antimicrobial resistance genes, plasmid replicon types and virulence genes using tools from the Center for Genomic Epidemiology.
E. coli strain MSF11 was serotype OUT:H10 and ST226. Acquired antimicrobial resistance genes [bla, qnrS1, catA2, mdf(A) and mcr-1.1] and virulence-related genes (astA and gad) were identified. The mcr-1 gene contained a single nucleotide polymorphism at position 27 (C→T) of the prototype, and the variant gene was associated with an IncX4-type plasmid. This plasmid-borne colistin resistance mediated by the mcr-1 variant has been observed among colistin-resistant strains from humans, animals and the environment previously reported in Thailand, although the STs and serotypes of the E. coli strains were different.
An mcr-1 variant was identified in an E. coli isolate harbouring the EAST1 (enteroaggregative E. coli heat-stable toxin 1) gene (astA) from a human diarrhoeal stool specimen. This study highlights the potential risk of dissemination of colistin-resistant E. coli in view of the prevalence of the variant gene on IncX4-type plasmids.
携带 mcr-1 基因的大肠杆菌分离株在腹泻患者中很少报道。在这里，我们报告了一株来自泰国住院急性腹泻患者的粘菌素耐药大肠杆菌的基因组序列草图。
从耐粘菌素的大肠杆菌分离株（MSF11）中提取全基因组 DNA，并使用 Ion Torrent 测序仪进行测序，测序方法采用 400-bp 读长化学法。使用基因组流行病学中心的工具，对 MSF11 的多位点序列型（ST）、血清型、获得性抗菌耐药基因、质粒复制子类型和毒力基因进行分析。
大肠杆菌菌株 MSF11 为 OUT:H10 和 ST226 血清型。鉴定出获得性抗菌耐药基因 [bla、qnrS1、catA2、mdf(A) 和 mcr-1.1] 和与毒力相关的基因（astA 和 gad）。mcr-1 基因在原型的 27 位（C→T）处发生了单核苷酸多态性，变异基因与 IncX4 型质粒有关。这种由 mcr-1 变异引起的粘菌素耐药已在泰国以前报道的耐粘菌素的人、动物和环境来源的大肠杆菌菌株中观察到，尽管大肠杆菌菌株的 ST 和血清型不同。
从人类腹泻粪便标本中分离出的一株携带 EAST1（肠聚集性大肠杆菌耐热毒素 1）基因（astA）的大肠杆菌中发现了 mcr-1 变异株。鉴于 IncX4 型质粒上变异基因的流行，本研究强调了耐粘菌素大肠杆菌传播的潜在风险。
