人类红细胞膜骨架可能是一种离子凝胶。III. 未肿胀红细胞的微量吸管吸取。

The human erythrocyte membrane skeleton may be an ionic gel. III. Micropipette aspiration of unswollen erythrocytes.

作者信息

Stokke B T, Mikkelsen A, Elgsaeter A

出版信息

J Theor Biol. 1986 Nov 21;123(2):205-11. doi: 10.1016/s0022-5193(86)80154-0.

Abstract

We have carried out a theoretical analysis of micropipette aspiration of unswollen erythrocytes using the protein-gel-lipid-bilayer membrane model and taking into account that the modulus of area compression of the membrane skeleton may depend on the environmental conditions. Our analysis shows that the aspiration pressure needed to obtain a certain membrane projection length is strongly dependent on the ratio between the membrane skeleton modulus of area compression and the elastic shear modulus. Our analysis therefore predicts that micropipette aspiration of unswollen erythrocytes may be a sensitive method for detection of changes in this ratio. The analysis thus also shows that micropipette aspiration of unswollen erythrocytes can not be used to determine the membrane shear modulus unless something is known about the membrane skeleton modulus of area compression.

摘要

我们使用蛋白质-凝胶-脂质双层膜模型，并考虑到膜骨架的面积压缩模量可能取决于环境条件，对未肿胀红细胞的微吸管抽吸进行了理论分析。我们的分析表明，获得一定膜突出长度所需的抽吸压力强烈依赖于膜骨架面积压缩模量与弹性剪切模量之间的比率。因此，我们的分析预测，未肿胀红细胞的微吸管抽吸可能是检测该比率变化的一种灵敏方法。该分析还表明，除非了解膜骨架的面积压缩模量，否则未肿胀红细胞的微吸管抽吸不能用于确定膜剪切模量。